Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

A. M. Dygai; E. G. Skurikhin; A. V. Pakhomova; A. A. Epanchintsev; O. V. Stronin; N. N. Ermakova; O. V. Pershina; L. A. Ermolaeva; V. A. Krupin; A. I. Kudryashova; V. V. Zhdanov

doi:10.1007/s10517-018-4232-5

Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

Authors: Dygai A.M.¹, Skurikhin E.G.¹, Pakhomova A.V.¹, Epanchintsev A.A.¹, Stronin O.V.¹, Ermakova N.N.¹, Pershina O.V.¹, Ermolaeva L.A.¹, Krupin V.A.¹, Kudryashova A.I.¹, Zhdanov V.V.¹
Affiliations:
1. Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Issue: Vol 165, No 5 (2018)
Pages: 644-648
Section: Pharmacology and Toxicology
URL: https://journals.rcsi.science/0007-4888/article/view/240626
DOI: https://doi.org/10.1007/s10517-018-4232-5
ID: 240626

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Abstract

The effects of the pegylated form of glucagon-like peptide 1 (pegGLP-1) on oligopotent β cell precursors (CD45^—TER119^—CD133⁺CD49f^low) in the pancreas were studied in C57Bl/6 mice. Under conditions of streptozotocin-induced type 1 diabetes mellitus, intraperitoneal injection of pegGLP1 increased the content of β cell precursors and dithizone-stained cells in the pancreas. β Cell precursors of mice with diabetes demonstrated high self-maintenance potential. In contrast to pegGLP-1, native GLP-1 did not affect β cell precursors in diabetic animals. Treatment of a culture of β cell precursors from mice with diabetes induced the yield of dithizone-stained mononuclears. In conditioned mediums of dithizone-positive cells obtained as a result of differentiation of β cell precursors from mice with diabetes, insulin was detected after administration of pegGLP-1 (10^—7 M) and glucose (3 mmol/liter); the level of insulin increased with increasing glucose concentration (to 20 mmol/liter). The in vitro effect of pegGLP-1 did not differ from the effect of GLP-1 (10^—7 M).

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Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

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Abstract

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