Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

A. M. Dygai; E. G. Skurikhin; A. V. Pakhomova; A. A. Epanchintsev; O. V. Stronin; N. N. Ermakova; O. V. Pershina; L. A. Ermolaeva; V. A. Krupin; A. I. Kudryashova; V. V. Zhdanov

doi:10.1007/s10517-018-4232-5

Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

作者: Dygai A.¹, Skurikhin E.¹, Pakhomova A.¹, Epanchintsev A.¹, Stronin O.¹, Ermakova N.¹, Pershina O.¹, Ermolaeva L.¹, Krupin V.¹, Kudryashova A.¹, Zhdanov V.¹
隶属关系:
1. Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
期: 卷 165, 编号 5 (2018)
页面: 644-648
栏目: Pharmacology and Toxicology
URL: https://journals.rcsi.science/0007-4888/article/view/240626
DOI: https://doi.org/10.1007/s10517-018-4232-5
ID: 240626

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The effects of the pegylated form of glucagon-like peptide 1 (pegGLP-1) on oligopotent β cell precursors (CD45^—TER119^—CD133⁺CD49f^low) in the pancreas were studied in C57Bl/6 mice. Under conditions of streptozotocin-induced type 1 diabetes mellitus, intraperitoneal injection of pegGLP1 increased the content of β cell precursors and dithizone-stained cells in the pancreas. β Cell precursors of mice with diabetes demonstrated high self-maintenance potential. In contrast to pegGLP-1, native GLP-1 did not affect β cell precursors in diabetic animals. Treatment of a culture of β cell precursors from mice with diabetes induced the yield of dithizone-stained mononuclears. In conditioned mediums of dithizone-positive cells obtained as a result of differentiation of β cell precursors from mice with diabetes, insulin was detected after administration of pegGLP-1 (10^—7 M) and glucose (3 mmol/liter); the level of insulin increased with increasing glucose concentration (to 20 mmol/liter). The in vitro effect of pegGLP-1 did not differ from the effect of GLP-1 (10^—7 M).

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Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

全文:

详细

作者简介

A. Dygai

E. Skurikhin

A. Pakhomova

A. Epanchintsev

O. Stronin

N. Ermakova

O. Pershina

L. Ermolaeva

V. Krupin

A. Kudryashova

V. Zhdanov