Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1
- Authors: Dygai A.M.1, Skurikhin E.G.1, Pakhomova A.V.1, Epanchintsev A.A.1, Stronin O.V.1, Ermakova N.N.1, Pershina O.V.1, Ermolaeva L.A.1, Krupin V.A.1, Kudryashova A.I.1, Zhdanov V.V.1
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Affiliations:
- Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
- Issue: Vol 165, No 5 (2018)
- Pages: 644-648
- Section: Pharmacology and Toxicology
- URL: https://journals.rcsi.science/0007-4888/article/view/240626
- DOI: https://doi.org/10.1007/s10517-018-4232-5
- ID: 240626
Cite item
Abstract
The effects of the pegylated form of glucagon-like peptide 1 (pegGLP-1) on oligopotent β cell precursors (CD45—TER119—CD133+CD49flow) in the pancreas were studied in C57Bl/6 mice. Under conditions of streptozotocin-induced type 1 diabetes mellitus, intraperitoneal injection of pegGLP1 increased the content of β cell precursors and dithizone-stained cells in the pancreas. β Cell precursors of mice with diabetes demonstrated high self-maintenance potential. In contrast to pegGLP-1, native GLP-1 did not affect β cell precursors in diabetic animals. Treatment of a culture of β cell precursors from mice with diabetes induced the yield of dithizone-stained mononuclears. In conditioned mediums of dithizone-positive cells obtained as a result of differentiation of β cell precursors from mice with diabetes, insulin was detected after administration of pegGLP-1 (10—7 M) and glucose (3 mmol/liter); the level of insulin increased with increasing glucose concentration (to 20 mmol/liter). The in vitro effect of pegGLP-1 did not differ from the effect of GLP-1 (10—7 M).
About the authors
A. M. Dygai
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
E. G. Skurikhin
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Author for correspondence.
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
A. V. Pakhomova
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
A. A. Epanchintsev
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
O. V. Stronin
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
N. N. Ermakova
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
O. V. Pershina
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
L. A. Ermolaeva
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
V. A. Krupin
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
A. I. Kudryashova
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk
V. V. Zhdanov
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Russian Federation, Tomsk