Interactions of NK Cells and Trophoblast Cells. Methodological Aspects
- Авторлар: Mikhailova V.1, Bazhenov D.1, Belikova M.2, Viknyanshchuk A.1, Kogan I.1, Sel’kov S.1, Sokolov D.1
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Мекемелер:
- D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- City Hospital No. 26
- Шығарылым: Том 165, № 4 (2018)
- Беттер: 548-553
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240508
- DOI: https://doi.org/10.1007/s10517-018-4212-9
- ID: 240508
Дәйексөз келтіру
Аннотация
NK cells present in different organs differ by their functional characteristics, in particular, proliferative activity. For studying tissue-resident NK cells, tissue-specific microenvironment should be reproduced. In case of decidual NK cells, this microenvironment is created by trophoblast cells. We developed a method for evaluation of proliferative activity of peripheral blood NK cells in the presence of trophoblast cells. Proliferative activity of peripheral blood NK cells was evaluated by the expression of protein Ki-67 after culturing with JEG-3 trophoblast cells. This method allows evaluating the functional state of NK cells in microenvironment specific for the decidua.
Авторлар туралы
V. Mikhailova
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Хат алмасуға жауапты Автор.
Email: mva_spb@mail.ru
Ресей, St. Petersburg
D. Bazhenov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Ресей, St. Petersburg
M. Belikova
City Hospital No. 26
Email: mva_spb@mail.ru
Ресей, St. Petersburg
A. Viknyanshchuk
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Ресей, St. Petersburg
I. Kogan
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Ресей, St. Petersburg
S. Sel’kov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Ресей, St. Petersburg
D. Sokolov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Ресей, St. Petersburg
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