Interactions of NK Cells and Trophoblast Cells. Methodological Aspects
- Authors: Mikhailova V.A.1, Bazhenov D.O.1, Belikova M.E.2, Viknyanshchuk A.N.1, Kogan I.Y.1, Sel’kov S.A.1, Sokolov D.I.1
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Affiliations:
- D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- City Hospital No. 26
- Issue: Vol 165, No 4 (2018)
- Pages: 548-553
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240508
- DOI: https://doi.org/10.1007/s10517-018-4212-9
- ID: 240508
Cite item
Abstract
NK cells present in different organs differ by their functional characteristics, in particular, proliferative activity. For studying tissue-resident NK cells, tissue-specific microenvironment should be reproduced. In case of decidual NK cells, this microenvironment is created by trophoblast cells. We developed a method for evaluation of proliferative activity of peripheral blood NK cells in the presence of trophoblast cells. Proliferative activity of peripheral blood NK cells was evaluated by the expression of protein Ki-67 after culturing with JEG-3 trophoblast cells. This method allows evaluating the functional state of NK cells in microenvironment specific for the decidua.
About the authors
V. A. Mikhailova
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Author for correspondence.
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
D. O. Bazhenov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
M. E. Belikova
City Hospital No. 26
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
A. N. Viknyanshchuk
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
I. Yu. Kogan
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
S. A. Sel’kov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
D. I. Sokolov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Russian Federation, St. Petersburg
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