Interactions of NK Cells and Trophoblast Cells. Methodological Aspects
- 作者: Mikhailova V.1, Bazhenov D.1, Belikova M.2, Viknyanshchuk A.1, Kogan I.1, Sel’kov S.1, Sokolov D.1
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隶属关系:
- D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- City Hospital No. 26
- 期: 卷 165, 编号 4 (2018)
- 页面: 548-553
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240508
- DOI: https://doi.org/10.1007/s10517-018-4212-9
- ID: 240508
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详细
NK cells present in different organs differ by their functional characteristics, in particular, proliferative activity. For studying tissue-resident NK cells, tissue-specific microenvironment should be reproduced. In case of decidual NK cells, this microenvironment is created by trophoblast cells. We developed a method for evaluation of proliferative activity of peripheral blood NK cells in the presence of trophoblast cells. Proliferative activity of peripheral blood NK cells was evaluated by the expression of protein Ki-67 after culturing with JEG-3 trophoblast cells. This method allows evaluating the functional state of NK cells in microenvironment specific for the decidua.
作者简介
V. Mikhailova
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
编辑信件的主要联系方式.
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
D. Bazhenov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
M. Belikova
City Hospital No. 26
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
A. Viknyanshchuk
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
I. Kogan
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
S. Sel’kov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
D. Sokolov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
俄罗斯联邦, St. Petersburg
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