Interactions of NK Cells and Trophoblast Cells. Methodological Aspects
- Autores: Mikhailova V.1, Bazhenov D.1, Belikova M.2, Viknyanshchuk A.1, Kogan I.1, Sel’kov S.1, Sokolov D.1
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Afiliações:
- D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- City Hospital No. 26
- Edição: Volume 165, Nº 4 (2018)
- Páginas: 548-553
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240508
- DOI: https://doi.org/10.1007/s10517-018-4212-9
- ID: 240508
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Resumo
NK cells present in different organs differ by their functional characteristics, in particular, proliferative activity. For studying tissue-resident NK cells, tissue-specific microenvironment should be reproduced. In case of decidual NK cells, this microenvironment is created by trophoblast cells. We developed a method for evaluation of proliferative activity of peripheral blood NK cells in the presence of trophoblast cells. Proliferative activity of peripheral blood NK cells was evaluated by the expression of protein Ki-67 after culturing with JEG-3 trophoblast cells. This method allows evaluating the functional state of NK cells in microenvironment specific for the decidua.
Sobre autores
V. Mikhailova
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Autor responsável pela correspondência
Email: mva_spb@mail.ru
Rússia, St. Petersburg
D. Bazhenov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Rússia, St. Petersburg
M. Belikova
City Hospital No. 26
Email: mva_spb@mail.ru
Rússia, St. Petersburg
A. Viknyanshchuk
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Rússia, St. Petersburg
I. Kogan
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Rússia, St. Petersburg
S. Sel’kov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Rússia, St. Petersburg
D. Sokolov
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: mva_spb@mail.ru
Rússia, St. Petersburg