Complex Analysis of Diffusion Transport and Microstructure of an Intervertebral Disk
- Authors: Byvaltsev V.A.1,2,3,4, Kolesnikov S.I.5,6, Belykh E.G.1,2, Stepanov I.A.1,2, Kalinin A.A.1,2, Bardonova L.A.1,2, Sudakov N.P.1,7, Klimenkov I.V.7, Nikiforov S.B.1, Semenov A.V.8, Perfil’ev D.V.8, Bespyatykh I.V.4, Antipina S.L.4, Giers M.1, Prul M.1
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Affiliations:
- Irkutsk Research Center of Surgery and Traumatology
- Irkutsk State Medical University
- Irkutsk State Medical Academy of Postgraduate Education
- Railway Clinical Hospital at Irkutsk Passengers Station
- Research Center of Problems of Family Health and Human Reproduction
- M. V. Lomonosov Moscow State University
- Limnological Institute, Siberian Division of the Russian Academy of Sciences
- Irkutsk Regional Forensic Medical Expert Bureau
- Issue: Vol 164, No 2 (2017)
- Pages: 223-228
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239522
- DOI: https://doi.org/10.1007/s10517-017-3963-z
- ID: 239522
Cite item
Abstract
We studied the relationship between diffusion transport and morphological and microstructural organization of extracellular matrix of human intervertebral disk. Specimens of the lumbar intervertebral disks without abnormalities were studied ex vivo by diffusion-weighed magnetic resonance imaging, histological and immunohistochemical methods, and electron microscopy. Distribution of the diffusion coefficient in various compartments of the intervertebral disk was studied. Significant correlations between diffusion coefficient and cell density in the nucleus pulposus, posterior aspects of annulus fibrosus, and endplate at the level of the posterior annulus fibrosus were detected for each disk. In disks with nucleus pulposus diffusion coefficient below 15×10-4 mm2/sec, collagens X and XI were detected apart from aggrecan and collagens I and II. The results supplement the concept on the relationship between the microstructure and cell composition of various compartments of the intervertebral disk and parameters of nutrient transport.
About the authors
V. A. Byvaltsev
Irkutsk Research Center of Surgery and Traumatology; Irkutsk State Medical University; Irkutsk State Medical Academy of Postgraduate Education; Railway Clinical Hospital at Irkutsk Passengers Station
Author for correspondence.
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk; Irkutsk; Irkutsk
S. I. Kolesnikov
Research Center of Problems of Family Health and Human Reproduction; M. V. Lomonosov Moscow State University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Moscow
E. G. Belykh
Irkutsk Research Center of Surgery and Traumatology; Irkutsk State Medical University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk
I. A. Stepanov
Irkutsk Research Center of Surgery and Traumatology; Irkutsk State Medical University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk
A. A. Kalinin
Irkutsk Research Center of Surgery and Traumatology; Irkutsk State Medical University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk
L. A. Bardonova
Irkutsk Research Center of Surgery and Traumatology; Irkutsk State Medical University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk
N. P. Sudakov
Irkutsk Research Center of Surgery and Traumatology; Limnological Institute, Siberian Division of the Russian Academy of Sciences
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk
I. V. Klimenkov
Limnological Institute, Siberian Division of the Russian Academy of Sciences
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
S. B. Nikiforov
Irkutsk Research Center of Surgery and Traumatology
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
A. V. Semenov
Irkutsk Regional Forensic Medical Expert Bureau
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
D. V. Perfil’ev
Irkutsk Regional Forensic Medical Expert Bureau
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
I. V. Bespyatykh
Railway Clinical Hospital at Irkutsk Passengers Station
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
S. L. Antipina
Railway Clinical Hospital at Irkutsk Passengers Station
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
M. Giers
Irkutsk Research Center of Surgery and Traumatology
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
M. Prul
Irkutsk Research Center of Surgery and Traumatology
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk