Email this article Application of Tetrameric Recombinant Human Butyrylcholinesterase as a Biopharmaceutical for Amelioration of Symptoms of Acute Organophosphate Poisoning
- Authors: Terekhov S.S.1, Palikov V.A.2, Palikova Y.A.2, Dyachenko I.A.2, Shamborant O.G.1, Smirnov I.V.1, Masson P.3, Gabibov A.G.1
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Affiliations:
- V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Affiliated Branch of V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Kazan (Volga Region) Federal University
- Issue: Vol 163, No 4 (2017)
- Pages: 430-435
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238965
- DOI: https://doi.org/10.1007/s10517-017-3821-z
- ID: 238965
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Abstract
We present a procedure for optimizing the expression of recombinant tetrameric butyrylcholinesterase that enables large-scale production with the yield >30 mg/liter (>90 mg/roller bottle). Intravenous injection of the preparation significantly increased survival and decreased the severity of symptoms of poisoning with paraoxon, an organophosphorus toxin.
About the authors
S. S. Terekhov
V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: sterekhoff@mail.ru
Russian Federation, Moscow
V. A. Palikov
Affiliated Branch of V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sterekhoff@mail.ru
Russian Federation, Pushchino, Moscow Region
Y. A. Palikova
Affiliated Branch of V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sterekhoff@mail.ru
Russian Federation, Pushchino, Moscow Region
I. A. Dyachenko
Affiliated Branch of V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sterekhoff@mail.ru
Russian Federation, Pushchino, Moscow Region
O. G. Shamborant
V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sterekhoff@mail.ru
Russian Federation, Moscow
I. V. Smirnov
V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sterekhoff@mail.ru
Russian Federation, Moscow
P. Masson
Kazan (Volga Region) Federal University
Email: sterekhoff@mail.ru
Russian Federation, Republic of Tatarstan, Kazan
A. G. Gabibov
V. V. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sterekhoff@mail.ru
Russian Federation, Moscow
