Email this article In Vitro Study of Cytophysiological Characteristics of Multinuclear Macrophages from Intact and BCG-Infected Mice
- Authors: Il’in D.A.1, Arkhipov S.A.1,2, Shkurupy V.A.1
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Affiliations:
- Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences
- Novosibirsk State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 5 (2016)
- Pages: 668-671
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236876
- DOI: https://doi.org/10.1007/s10517-016-3245-1
- ID: 236876
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Abstract
Peritoneal macrophages were isolated from intact and BCG-infected BALB/c mice and explanted in vitro. Multinuclear macrophages formed in these cultures differed by the number of nuclei, expression of apoptosis inductors and regulators (TNF-α, p53 protein, caspase 3, and Bcl-2 protein), and cytophysiological characteristics (phagocytic activity, ROS generation, and antimycobacterial properties). Our results indicate that the formation of multinuclear macrophages is accompanied by induction of apoptosis (p53 signaling pathway) and appearance of multinuclear macrophage-derived cells characterized by high phagocytic and antimycobacterial activity.
About the authors
D. A. Il’in
Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences
Email: arkhipov@centercem.ru
Russian Federation, Tomsk
S. A. Arkhipov
Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences; Novosibirsk State Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: arkhipov@centercem.ru
Russian Federation, Tomsk; Novosibirsk
V. A. Shkurupy
Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences
Email: arkhipov@centercem.ru
Russian Federation, Tomsk
