Enviar artigo por via de e-mail In Vitro Study of Cytophysiological Characteristics of Multinuclear Macrophages from Intact and BCG-Infected Mice
- Autores: Il’in D.1, Arkhipov S.1,2, Shkurupy V.1
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Afiliações:
- Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences
- Novosibirsk State Medical University, Ministry of Health of the Russian Federation
- Edição: Volume 160, Nº 5 (2016)
- Páginas: 668-671
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236876
- DOI: https://doi.org/10.1007/s10517-016-3245-1
- ID: 236876
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Resumo
Peritoneal macrophages were isolated from intact and BCG-infected BALB/c mice and explanted in vitro. Multinuclear macrophages formed in these cultures differed by the number of nuclei, expression of apoptosis inductors and regulators (TNF-α, p53 protein, caspase 3, and Bcl-2 protein), and cytophysiological characteristics (phagocytic activity, ROS generation, and antimycobacterial properties). Our results indicate that the formation of multinuclear macrophages is accompanied by induction of apoptosis (p53 signaling pathway) and appearance of multinuclear macrophage-derived cells characterized by high phagocytic and antimycobacterial activity.
Sobre autores
D. Il’in
Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences
Email: arkhipov@centercem.ru
Rússia, Tomsk
S. Arkhipov
Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences; Novosibirsk State Medical University, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: arkhipov@centercem.ru
Rússia, Tomsk; Novosibirsk
V. Shkurupy
Research Institute of Experimental and Clinical Medicine, Siberian Division of the Russian Academy of Medical Sciences
Email: arkhipov@centercem.ru
Rússia, Tomsk
