Study of the tissue distribution of potential boron neutron-capture therapy agents based on conjugates of chlorin  e  6  aminoamide derivatives with boron nanoparticles

A. B. Volovetskiy; N. Y. Shilyagina; V. V. Dudenkova; S. O. Pasynkova; A. A. Ignatova; A. F. Mironov; M. A. Grin; V. I. Bregadze; A. V. Feofanov; I. V. Balalaeva; A. V. Maslennikova

doi:10.1134/S0006350916010255

Study of the tissue distribution of potential boron neutron-capture therapy agents based on conjugates of chlorin e₆ aminoamide derivatives with boron nanoparticles

Authors: Volovetskiy A.B.¹, Shilyagina N.Y.¹, Dudenkova V.V.¹, Pasynkova S.O.², Ignatova A.A.³^,4, Mironov A.F.², Grin M.A.², Bregadze V.I.⁵, Feofanov A.V.³^,4, Balalaeva I.V.¹, Maslennikova A.V.¹^,6
Affiliations:
1. Lobachevsky State University of Nizhny Novgorod
2. Moscow State University of Fine Chemical Technologies
3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
4. Moscow State University
5. Nesmeyanov Institute of Organoelement Compounds
6. Nizhny Novgorod State Medical Academy
Issue: Vol 61, No 1 (2016)
Pages: 133-138
Section: Biophysics of Complex Systems
URL: https://journals.rcsi.science/0006-3509/article/view/151928
DOI: https://doi.org/10.1134/S0006350916010255
ID: 151928

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Abstract

Boron neutron-capture therapy of cancer is based on the ability of the ¹⁰B isotope to capture thermal neutrons; it is one of the most promising techniques in radiation therapy. The high content and selective accumulation of ¹⁰B in the tumor tissue are the most important prerequisites for its efficacy. The purpose of this study was to determine the biodistribution of cobalt bis(dicarbollide) conjugates with chlorin e₆ amino amide derivatives. Experiments were carried out in Balb/c mice with transplanted CT-26 murine colon carcinoma. Boron-containing conjugates were injected into the tail vein at a dose of 10 mg/kg. The conjugate accumulation in tumor tissue and organs was studied by laser scanning microscopy. Excitation was performed at the wavelength of 514 nm; the signals were recorded in the range of 560–710 nm in increments of 10 nm. To evaluate the amount of the boron conjugate, we calculated the intensity of the fluorescence signal of the samples under investigation. At 3 h after administration of the agent, a high level of fluorescence was observed in the liver, spleen, and lung. The tumor/muscle accumulation ratio was approximately 3. The study demonstrated that boron derivatives of chlorin e₆ are promising agents for boron neutron-capture therapy.

Keywords

boron neutron-capture therapy, chlorin e₆, photosensitizer, laser scanning microscopy

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Study of the tissue distribution of potential boron neutron-capture therapy agents based on conjugates of chlorin e6 aminoamide derivatives with boron nanoparticles

Full Text

Abstract

Keywords

About the authors

Supplementary files

Study of the tissue distribution of potential boron neutron-capture therapy agents based on conjugates of chlorin e₆ aminoamide derivatives with boron nanoparticles