Отправить статью по E-mail Structural Study of the Complex Formed by Ceruloplasmin and Macrophage Migration Inhibitory Factor
- Авторы: Sokolov A.1,2,3,4, Dadinova L.5, Petoukhov M.5,6,7,8, Bourenkov G.6, Dubova K.5, Amarantov S.5, Volkov V.5, Kostevich V.1,2, Gorbunov N.1, Grudinina N.1, Vasilyev V.1,3, Samygina V.5,9
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Учреждения:
- Institute of Experimental Medicine
- Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
- St. Petersburg State University
- Center of Preclinical Translational Research
- Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
- European Molecular Biology Laboratory (EMBL)
- Frumkin Institute of Physical Chemistry and Electrochemistry
- Semenov Institute of Chemical Physics
- National Research Center “Kurchatov Institute”
- Выпуск: Том 83, № 6 (2018)
- Страницы: 701-707
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151672
- DOI: https://doi.org/10.1134/S000629791806007X
- ID: 151672
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Аннотация
Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine. Inhibitors of tautomerase activity of MIF are perspective antiinflammatory compounds. Ceruloplasmin, the copper-containing ferroxidase of blood plasma, is a noncompetitive inhibitor of tautomerase activity of MIF in the reaction with p-hydroxyphenylpyruvate. Small-angle X-ray scattering established a model of the complex formed by MIF and ceruloplasmin. Crystallographic analysis of MIF with a modified active site supports the model. The stoichiometry of 3 CP/MIF trimer complex was established using gel filtration. Conformity of novel data concerning the interaction regions in the studied proteins with previous biochemical data is discussed.
Об авторах
A. Sokolov
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency; St. Petersburg State University; Center of Preclinical Translational Research
Email: lera@crys.ras.ru
Россия, St. Petersburg, 197376; Moscow, 119435; St. Petersburg, 199000; St. Petersburg, 197371
L. Dadinova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Россия, Moscow, 119333
M. Petoukhov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; European Molecular Biology Laboratory (EMBL); Frumkin Institute of Physical Chemistry and Electrochemistry; Semenov Institute of Chemical Physics
Email: lera@crys.ras.ru
Россия, Moscow, 119333; Hamburg, 22607; Moscow, 119071; Moscow, 119991
G. Bourenkov
European Molecular Biology Laboratory (EMBL)
Email: lera@crys.ras.ru
Германия, Hamburg, 22607
K. Dubova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Россия, Moscow, 119333
S. Amarantov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Россия, Moscow, 119333
V. Volkov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Россия, Moscow, 119333
V. Kostevich
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: lera@crys.ras.ru
Россия, St. Petersburg, 197376; Moscow, 119435
N. Gorbunov
Institute of Experimental Medicine
Email: lera@crys.ras.ru
Россия, St. Petersburg, 197376
N. Grudinina
Institute of Experimental Medicine
Email: lera@crys.ras.ru
Россия, St. Petersburg, 197376
V. Vasilyev
Institute of Experimental Medicine; St. Petersburg State University
Email: lera@crys.ras.ru
Россия, St. Petersburg, 197376; St. Petersburg, 199000
V. Samygina
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; National Research Center “Kurchatov Institute”
Автор, ответственный за переписку.
Email: lera@crys.ras.ru
Россия, Moscow, 119333; Moscow, 123182
