电邮这篇文章 Structural Study of the Complex Formed by Ceruloplasmin and Macrophage Migration Inhibitory Factor
- 作者: Sokolov A.1,2,3,4, Dadinova L.5, Petoukhov M.5,6,7,8, Bourenkov G.6, Dubova K.5, Amarantov S.5, Volkov V.5, Kostevich V.1,2, Gorbunov N.1, Grudinina N.1, Vasilyev V.1,3, Samygina V.5,9
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隶属关系:
- Institute of Experimental Medicine
- Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
- St. Petersburg State University
- Center of Preclinical Translational Research
- Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
- European Molecular Biology Laboratory (EMBL)
- Frumkin Institute of Physical Chemistry and Electrochemistry
- Semenov Institute of Chemical Physics
- National Research Center “Kurchatov Institute”
- 期: 卷 83, 编号 6 (2018)
- 页面: 701-707
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151672
- DOI: https://doi.org/10.1134/S000629791806007X
- ID: 151672
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详细
Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine. Inhibitors of tautomerase activity of MIF are perspective antiinflammatory compounds. Ceruloplasmin, the copper-containing ferroxidase of blood plasma, is a noncompetitive inhibitor of tautomerase activity of MIF in the reaction with p-hydroxyphenylpyruvate. Small-angle X-ray scattering established a model of the complex formed by MIF and ceruloplasmin. Crystallographic analysis of MIF with a modified active site supports the model. The stoichiometry of 3 CP/MIF trimer complex was established using gel filtration. Conformity of novel data concerning the interaction regions in the studied proteins with previous biochemical data is discussed.
作者简介
A. Sokolov
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency; St. Petersburg State University; Center of Preclinical Translational Research
Email: lera@crys.ras.ru
俄罗斯联邦, St. Petersburg, 197376; Moscow, 119435; St. Petersburg, 199000; St. Petersburg, 197371
L. Dadinova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
俄罗斯联邦, Moscow, 119333
M. Petoukhov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; European Molecular Biology Laboratory (EMBL); Frumkin Institute of Physical Chemistry and Electrochemistry; Semenov Institute of Chemical Physics
Email: lera@crys.ras.ru
俄罗斯联邦, Moscow, 119333; Hamburg, 22607; Moscow, 119071; Moscow, 119991
G. Bourenkov
European Molecular Biology Laboratory (EMBL)
Email: lera@crys.ras.ru
德国, Hamburg, 22607
K. Dubova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
俄罗斯联邦, Moscow, 119333
S. Amarantov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
俄罗斯联邦, Moscow, 119333
V. Volkov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
俄罗斯联邦, Moscow, 119333
V. Kostevich
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: lera@crys.ras.ru
俄罗斯联邦, St. Petersburg, 197376; Moscow, 119435
N. Gorbunov
Institute of Experimental Medicine
Email: lera@crys.ras.ru
俄罗斯联邦, St. Petersburg, 197376
N. Grudinina
Institute of Experimental Medicine
Email: lera@crys.ras.ru
俄罗斯联邦, St. Petersburg, 197376
V. Vasilyev
Institute of Experimental Medicine; St. Petersburg State University
Email: lera@crys.ras.ru
俄罗斯联邦, St. Petersburg, 197376; St. Petersburg, 199000
V. Samygina
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; National Research Center “Kurchatov Institute”
编辑信件的主要联系方式.
Email: lera@crys.ras.ru
俄罗斯联邦, Moscow, 119333; Moscow, 123182
