Мақаланы E-mail арқылы жіберу Structural Study of the Complex Formed by Ceruloplasmin and Macrophage Migration Inhibitory Factor
- Авторлар: Sokolov A.1,2,3,4, Dadinova L.5, Petoukhov M.5,6,7,8, Bourenkov G.6, Dubova K.5, Amarantov S.5, Volkov V.5, Kostevich V.1,2, Gorbunov N.1, Grudinina N.1, Vasilyev V.1,3, Samygina V.5,9
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Мекемелер:
- Institute of Experimental Medicine
- Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
- St. Petersburg State University
- Center of Preclinical Translational Research
- Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
- European Molecular Biology Laboratory (EMBL)
- Frumkin Institute of Physical Chemistry and Electrochemistry
- Semenov Institute of Chemical Physics
- National Research Center “Kurchatov Institute”
- Шығарылым: Том 83, № 6 (2018)
- Беттер: 701-707
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151672
- DOI: https://doi.org/10.1134/S000629791806007X
- ID: 151672
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine. Inhibitors of tautomerase activity of MIF are perspective antiinflammatory compounds. Ceruloplasmin, the copper-containing ferroxidase of blood plasma, is a noncompetitive inhibitor of tautomerase activity of MIF in the reaction with p-hydroxyphenylpyruvate. Small-angle X-ray scattering established a model of the complex formed by MIF and ceruloplasmin. Crystallographic analysis of MIF with a modified active site supports the model. The stoichiometry of 3 CP/MIF trimer complex was established using gel filtration. Conformity of novel data concerning the interaction regions in the studied proteins with previous biochemical data is discussed.
Авторлар туралы
A. Sokolov
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency; St. Petersburg State University; Center of Preclinical Translational Research
Email: lera@crys.ras.ru
Ресей, St. Petersburg, 197376; Moscow, 119435; St. Petersburg, 199000; St. Petersburg, 197371
L. Dadinova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Ресей, Moscow, 119333
M. Petoukhov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; European Molecular Biology Laboratory (EMBL); Frumkin Institute of Physical Chemistry and Electrochemistry; Semenov Institute of Chemical Physics
Email: lera@crys.ras.ru
Ресей, Moscow, 119333; Hamburg, 22607; Moscow, 119071; Moscow, 119991
G. Bourenkov
European Molecular Biology Laboratory (EMBL)
Email: lera@crys.ras.ru
Германия, Hamburg, 22607
K. Dubova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Ресей, Moscow, 119333
S. Amarantov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Ресей, Moscow, 119333
V. Volkov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Ресей, Moscow, 119333
V. Kostevich
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: lera@crys.ras.ru
Ресей, St. Petersburg, 197376; Moscow, 119435
N. Gorbunov
Institute of Experimental Medicine
Email: lera@crys.ras.ru
Ресей, St. Petersburg, 197376
N. Grudinina
Institute of Experimental Medicine
Email: lera@crys.ras.ru
Ресей, St. Petersburg, 197376
V. Vasilyev
Institute of Experimental Medicine; St. Petersburg State University
Email: lera@crys.ras.ru
Ресей, St. Petersburg, 197376; St. Petersburg, 199000
V. Samygina
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; National Research Center “Kurchatov Institute”
Хат алмасуға жауапты Автор.
Email: lera@crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123182
