Email this article Structural Study of the Complex Formed by Ceruloplasmin and Macrophage Migration Inhibitory Factor
- Authors: Sokolov A.V.1,2,3,4, Dadinova L.A.5, Petoukhov M.V.5,6,7,8, Bourenkov G.6, Dubova K.M.5, Amarantov S.V.5, Volkov V.V.5, Kostevich V.A.1,2, Gorbunov N.P.1, Grudinina N.A.1, Vasilyev V.B.1,3, Samygina V.R.5,9
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Affiliations:
- Institute of Experimental Medicine
- Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
- St. Petersburg State University
- Center of Preclinical Translational Research
- Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
- European Molecular Biology Laboratory (EMBL)
- Frumkin Institute of Physical Chemistry and Electrochemistry
- Semenov Institute of Chemical Physics
- National Research Center “Kurchatov Institute”
- Issue: Vol 83, No 6 (2018)
- Pages: 701-707
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151672
- DOI: https://doi.org/10.1134/S000629791806007X
- ID: 151672
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Abstract
Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine. Inhibitors of tautomerase activity of MIF are perspective antiinflammatory compounds. Ceruloplasmin, the copper-containing ferroxidase of blood plasma, is a noncompetitive inhibitor of tautomerase activity of MIF in the reaction with p-hydroxyphenylpyruvate. Small-angle X-ray scattering established a model of the complex formed by MIF and ceruloplasmin. Crystallographic analysis of MIF with a modified active site supports the model. The stoichiometry of 3 CP/MIF trimer complex was established using gel filtration. Conformity of novel data concerning the interaction regions in the studied proteins with previous biochemical data is discussed.
About the authors
A. V. Sokolov
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency; St. Petersburg State University; Center of Preclinical Translational Research
Email: lera@crys.ras.ru
Russian Federation, St. Petersburg, 197376; Moscow, 119435; St. Petersburg, 199000; St. Petersburg, 197371
L. A. Dadinova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Russian Federation, Moscow, 119333
M. V. Petoukhov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; European Molecular Biology Laboratory (EMBL); Frumkin Institute of Physical Chemistry and Electrochemistry; Semenov Institute of Chemical Physics
Email: lera@crys.ras.ru
Russian Federation, Moscow, 119333; Hamburg, 22607; Moscow, 119071; Moscow, 119991
G. Bourenkov
European Molecular Biology Laboratory (EMBL)
Email: lera@crys.ras.ru
Germany, Hamburg, 22607
K. M. Dubova
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Russian Federation, Moscow, 119333
S. V. Amarantov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Russian Federation, Moscow, 119333
V. V. Volkov
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”
Email: lera@crys.ras.ru
Russian Federation, Moscow, 119333
V. A. Kostevich
Institute of Experimental Medicine; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: lera@crys.ras.ru
Russian Federation, St. Petersburg, 197376; Moscow, 119435
N. P. Gorbunov
Institute of Experimental Medicine
Email: lera@crys.ras.ru
Russian Federation, St. Petersburg, 197376
N. A. Grudinina
Institute of Experimental Medicine
Email: lera@crys.ras.ru
Russian Federation, St. Petersburg, 197376
V. B. Vasilyev
Institute of Experimental Medicine; St. Petersburg State University
Email: lera@crys.ras.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 199000
V. R. Samygina
Shubnikov Institute of Crystallography, FSRC “Crystallography and Photonics”; National Research Center “Kurchatov Institute”
Author for correspondence.
Email: lera@crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123182
