Features of the epithelial layer formation on corneal graft

封面

如何引用文章

全文:

详细

Relevance. Significant impact on graft survival has a delay in the formation of epithelial cover.

Purpose – cytological characteristics and analysis of the effect of medications on the epithelial layer formation on the graft.

Materials and methods. An analysis of the post-operative state of 20 patients operated for bullous keratopathy and corneal ulcer. Smears from the surface of the graft were stained by Leucodif 200 (LDF 200) and examined by light microscopy.

Results. The epithelial layer formation in all cases ended no later than 8-10 days after keratoplasty. According to the cytological analysis, a complex of postoperative treatment which included Vixipin from the 10th day of the postoperative period. Visual acuity improvement occurred in 11 patients (75% of cases) operated due for bullous keratopathy, and in four patients operated for corneal ulcer.

Conclusions. The epithelial layer formation on the surface of corneal transplant occurs due to the migration of mature epithelial cells from the wound edges of the own cornea and mitotic activity of limbal cells of the recipient. Long-lasting epithelial defect and inflammation preceding keratoplasty result in slower epithelialization of the graft in the postoperative period. Use of corticosteroids, sodium hyaluronate and antioxidants in the postoperative period contribute to the formation of a full epithelial layer.

作者简介

Evgeniy Miludin

GBUZ Samara Regional Clinical Ophthalmologic Hospital

编辑信件的主要联系方式.
Email: miljudin@mail.ru
ORCID iD: 0000-0001-7610-7523
SPIN 代码: 2034-2910
Scopus 作者 ID: 7801402280

д-р мед. наук, заведующий отделением, врач-офтальмолог высшей категории

俄罗斯联邦, Samara

Kseniya Kuchuk

Samara State Medical University

Email: kuchukke@rambler.ru
SPIN 代码: 7785-4779

Ophthalmologist

俄罗斯联邦, Samara

参考

  1. Слонимский А.Ю., Слонимский Ю.Б., Долгий С.С. Сквозная пересадка роговицы при гнойных процессах переднего отрезка глазного яблока // РМЖ. Клиническая офтальмология. – 2010. – Т. 11. – № 1. – С. 11–14. [Slonimskii AY, Slonimskii YB, Dolgii SS. Penetrating keratoplasty in patients with purulent processes of anterior eye segment. RMZh. Klinicheskaya oftalmologiya. 2010;11(1):11-14. (In Russ.)]
  2. Злобин И.А., Черныш В.Ф., Чурашов С.В., и др. Роль импрессионной цитологии в оценке фенотипа эпителия роговицы при решении вопроса о показаниях к операции оптической кератопластики // Вестник Российской военно-медицинской академии. – 2016. – № 1. – С. 89–93. [Zlobin IA, Chernysh VF, Churashov SV, et al. Role of impression cytology in cornea epithelium phenotype assessment at solution of question of indications to operation of optical keratoplasty. Vestnik Rossiiskoi voenno-meditsinskoi akademii. 2016;1:89-93. (In Russ.)]
  3. Милюдин Е.С., Золотарёв А.В., Милюдин А.Е. Возможности местной иммуносупрессии при повторной кератопластике // РМЖ. Клиническая офтальмология. – 2014. – Т. 14. – № 3. – С. 1–5. [Milyudin ES, Zolotarev AV, Milyudin AE. Possibilities of local immunosuppression in repeated keratoplasty. RMZh. Klinicheskaya oftalmologiya. 2014;14(3):1-5. (In Russ.)]
  4. Тонаева Х.Д., Онищенко Н.А., Борзенок С.А. Лимбальная трансплантация как способ индукции локальной иммунной толерантности при кератопластике высокого риска // Вестник трансплантологии и искусственных органов. – 2011. – Т. 13. – № 2. – С. 95–102. [Tonaeva KD, Onishchenko NA, Borzenok SA. Limbal transplantation as a method of local immune tolerance induction in high risk keratoplasty. Russian Journal of Transplantology and Artificial Organs. 2011;13(2):95-102. (In Russ.)]. https://doi.org/10.15825/1995-1191-2011-2-95-102.
  5. Feiz V, Mannis MJ, Kandavel G, et al. Surface keratopathy after penetrating keratoplasty. Trans Am Ophthalmol Soc. 2001;99:159-168.
  6. Tsubota K, Mashima Y, Murata H, et al. Corneal epithelium following penetrating keratoplasty. Br J Ophthalmol. 1995;79(3):257-260. https://doi.org/10.1136/bjo.79.3.257.
  7. Raj A, Dhasmana R, Bahadur H. Factors associated with surface epithelial keratopathy after optical penetrating keratoplasty. J Curr Ophthalmol. 2017;29(2):108-115. https://doi.org/10.1016/j.joco.2017.01.002.
  8. Борзенок С.А. Медико-технологические и методологические основы эффективной деятельности глазных тканевых банков России в обеспечении операций по сквозной трансплантации роговицы: Дис. … д-ра мед. наук. – М., 2008. – С. 240–259. [Borzenok SA. Mediko-tekhnologicheskiye i metodologicheskiye osnovy razvitiya deyatel’nosti glaznykh tkanevykh bankov Rossii v obespechenii operatsiy po skvoznoy transplantatsii rogovitsy. [dissertation] Moscow; 2008. P. 240-259. (In Russ.)]
  9. Труфанов С.В., Маложен С.А., Полунина Е.Г., и др. Синдром рецидивирующей эрозии роговицы (обзор) // Офтальмология. – 2015. – Т. 12. – № 2. – С. 4–12. [Trufanov SV, Malozhen SA, Polunina EG, et al. Recurrent corneal erosion syndrome (a review). Ophthalmology in Russia. 2015;12(2):4-12. (In Russ.)]. https://doi.org/10.18008/1816-5095-2015-2-4-12.
  10. Garrana RM, Zieske JD, Assouline M, Gipson IK. Matrix metalloproteinases in epithelia from human recurrent corneal erosion. Invest Ophthalmol Vis Sci. 1999;40(6):1266-1270.
  11. Feiz V, Mannis MJ, Kandavel G, et al. Surface keratopathy after penetrating keratoplasty. Trans Am Ophthalmol Soc. 2001;99:159-168.
  12. Smolin G. Suppression of the Corneal Homograft Reaction by Antilymphocyte Serum. Arch Ophthalmol. 1969;81(4):571-576. https://doi.org/10.1001/archopht.1969.00990010573020.
  13. Vannas A, Holden BA, Sweeney DF. Epithelial metabolism of the corneal graft is abnormal. Br J Ophthalmol. 1987;71(8):593-597. https://doi.org/10.1136/bjo.71.8.593.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Miludin E.S., Kuchuk K.E., 2019

Creative Commons License
此作品已接受知识共享署名 4.0国际许可协议的许可
 


##common.cookie##