Features of the epithelial layer formation on corneal graft

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Abstract

Relevance. Significant impact on graft survival has a delay in the formation of epithelial cover.

Purpose – cytological characteristics and analysis of the effect of medications on the epithelial layer formation on the graft.

Materials and methods. An analysis of the post-operative state of 20 patients operated for bullous keratopathy and corneal ulcer. Smears from the surface of the graft were stained by Leucodif 200 (LDF 200) and examined by light microscopy.

Results. The epithelial layer formation in all cases ended no later than 8-10 days after keratoplasty. According to the cytological analysis, a complex of postoperative treatment which included Vixipin from the 10th day of the postoperative period. Visual acuity improvement occurred in 11 patients (75% of cases) operated due for bullous keratopathy, and in four patients operated for corneal ulcer.

Conclusions. The epithelial layer formation on the surface of corneal transplant occurs due to the migration of mature epithelial cells from the wound edges of the own cornea and mitotic activity of limbal cells of the recipient. Long-lasting epithelial defect and inflammation preceding keratoplasty result in slower epithelialization of the graft in the postoperative period. Use of corticosteroids, sodium hyaluronate and antioxidants in the postoperative period contribute to the formation of a full epithelial layer.

About the authors

Evgeniy S. Miludin

GBUZ Samara Regional Clinical Ophthalmologic Hospital

Author for correspondence.
Email: miljudin@mail.ru
ORCID iD: 0000-0001-7610-7523
SPIN-code: 2034-2910
Scopus Author ID: 7801402280

д-р мед. наук, заведующий отделением, врач-офтальмолог высшей категории

Russian Federation, Samara

Kseniya E. Kuchuk

Samara State Medical University

Email: kuchukke@rambler.ru
SPIN-code: 7785-4779

Ophthalmologist

Russian Federation, Samara

References

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Supplementary files

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2. Fig. 1. Patient A. Diagnosis: Bullous keratopathy of the right eye. Second day after penetrating keratoplasty. Leucodif 200 (LDF 200) stain. Magnification ×400

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3. Fig. 2. Patient R. Diagnosis: Corneal ulcer of the left eye. Second day after penetrating keratoplasty. Leucodif 200 (LDF 200) stain. Magnification ×400

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4. Fig. 3. Patient R. Diagnosis: Bullous keratopathy of the right eye. 10th day after penetrating keratoplasty. Leucodif 200 (LDF 200) stain. Magnification ×400

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5. Fig. 4. Patient N. Diagnosis: Corneal ulcer of the left eye. 10th day after penetrating keratoplasty. Leucodif 200 (LDF 200) stain. Magnification ×400

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6. Fig. 5. Patient R. Diagnosis: Bullous keratopathy. 90 days after penetrating keratoplasty. Leucodif 200 (LDF 200) stain. Magnification ×400

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Copyright (c) 2019 Miludin E.S., Kuchuk K.E.

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