The use of platelet-rich plasma and two-component autofibrin glue in treatment of experimental chronic persistent corneal erosion

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Abstract

BACKGROUND: The use of platelet-rich plasma (PRP) and its modifications are a promising treatment method for chronic inflammatory diseases of the cornea.

AIM: To evaluate and compare the clinical and morphological features of the corneal regeneration during experimental chronic persistent corneal erosion with the use of PRP and two-component autofibrin glue (TAFG).

MATERIALS AND METHODS: The studies were conducted in 18 rabbits (36 eyes). After modeling chronic persistent corneal erosion, the animals of the 1st group were treated with TAFG; animals of the 2nd group — with instillations of PRP, animals of the 3rd group — with standard medical therapy.

RESULTS: By Day 14 of the experiment, the restoration of the transparency of the cornea, no erosion and new vessels in animals of the 1st and 2nd group were noted. This pattern persisted until Day 60. By Day 30 of the experiment, the presence of corneal clouding in optical zone, neovascularization over the whole cornea and an epithelial defect (3,85 [3,50; 4,47] %) in animals of the 3rd group were recorded. By Day 60, a complete epithelialization of the cornea was observed.

CONCLUSIONS: The use of PRP and TAFG allows reducing the time of corneal epithelialization by 2,5 times in conditions of experimental chronic persistent corneal erosion. There were no significant differences between the use of PRP (within 5 days) and TAFG, however, treatment with an adhesive composition is more convenient for both the doctor and the patient — a single use that does not require multiple use of expensive consumable materials.

About the authors

Violetta A. Toloknova

Kirov Military Medical Academy

Author for correspondence.
Email: toloknova.violet@mail.ru

student of the residency in the specialty “ophthalmology”

Russian Federation, Saint Petersburg

Ilya O. Gavriluk

Kirov Military Medical Academy

Email: iluaayaks@gmail.com
ORCID iD: 0000-0003-0932-2818
SPIN-code: 9649-7311

Cand. Sci. (Med.), Lecturer of the Department of Ophthalmology

Russian Federation, Saint Petersburg

Sergey V. Churashov

Kirov Military Medical Academy

Email: churashoff@mail.ru
ORCID iD: 0000-0003-1197-9237
SPIN-code: 5370-7410

Dr. Sci. (Med.), Professor of the Department of Ophthalmology

Russian Federation, Saint Petersburg

Alexei N. Kulikov

Kirov Military Medical Academy

Email: alexey.kulikov@mail.ru
ORCID iD: 0000-0002-5274-6993
SPIN-code: 6440-7706
Scopus Author ID: 198153
ResearcherId: M-2094-2016

Dr. Sci. (Med.), Chief of the Department of Ophthalmology

Russian Federation, Saint Petersburg

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Supplementary files

Supplementary Files
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2. Fig. 1. Biomicroscopic picture of the cornea in animals of the 1st group, Day 30: no clouding, no new vessels, a complete epithelialization of the cornea

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3. Fig. 2. Biomicroscopic picture of the cornea in animals of the 2nd group, Day 30: no clouding, no new vessels, a complete epithelialization of the cornea

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4. Fig. 3. Biomicroscopic picture of the cornea in animals of the 3rd group, Day 30: no corneal clouding in optical zone, neovascularization over the whole cornea, corneal erosion

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5. Fig. 4. Statistical analysis of corneal clouding dynamics. The Kruskal–Wallis test. * p < 0.05 when compared with the 1st group; ** p < 0.05 when compared with the 2nd group; # p < 0.05 when compared with the 3rd (control) group

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6. Fig. 5. Statistical analysis of corneal clouding dynamics. The Kruskal–Wallis test. * p < 0.05 when compared with the 1st group; ** p < 0.05 when compared with the 2nd group; # p < 0.05 when compared with the 3rd (control) group

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7. Fig. 6. Statistical analysis of corneal clouding dynamics. The Kruskal–Wallis test. # p < 0.05 when compared with the 3rd (control) group

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8. Fig. 7. Histologic study of the cornea in animals, Day 30: a — of the 1st and 2nd group; b — of the 3rd group. Hematoxylin and eosin staining (×200)

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