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Post a Comment The use of platelet lysate to increase the growth-stimulating effect of the amniotic membrane in vitro
- Authors: Bozhenko D.A.1, Chentsova E.V.1, Borovkova N.V.2, Ponomarev I.N.2, Storozheva M.V.2, Makarov M.S.2, Makarov P.V.1
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Affiliations:
- Helmholtz National Medical Research Center for Eye Diseases
- N.V. Sklifosovsky Research Institute for Emergency Medicine
- Issue: Vol 15, No 3 (2022)
- Pages: 57-62
- Section: Experimental trials
- URL: https://journals.rcsi.science/ov/article/view/131577
- DOI: https://doi.org/10.17816/OV108704
- ID: 131577
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Abstract
AIM: To work out the technique of saturation of the preserved amniotic membrane (AM) with platelet rich plasma (PRP) lysate and to evaluate the growth-stimulating effect of a combination of AM and PRP lysate in vitro.
MATERIALS AND METHODS: In the experiment, AM samples preserved in 3 ways were used: silicate drying, lyophilization, cryopreservation. PRP lysate was prepared on the basis of volunteers’ blood. During the exposure of AM with PRP lysate, the optimal saturation time of canned AM with lysate was determined, the volume of lysate that 1 cm2 of AM could adsorb was estimated. The growth-stimulating effect of AM transplants was evaluated in the culture of human buccal epithelium. The dynamics of cell growth was evaluated after 1, 2 and 3 days from the moment of sowing.
RESULTS: In the presence of PRP lysate, the mass of silicate–dried AM increased 4.2 times, lyophilized AM — 4.8 times, cryopreserved AM — 1.8 times. AM samples obtained by lyophilization adsorbed PRP lysate most effectively. Five minutes of exposure with PRP lysate are enough to fully saturate the AM. AM without PRP lysate did not give a growth-stimulating effect.
CONCLUSIONS: When comparing experiments with PRP lysate without AM and AM with PRP lysate, it was found that the greatest stimulation of cell growth occurred when using lyophilized AM and PRP lysate. Saturation of cryopreserved AM with PRP lysate was ineffective, and when using silicate-dried AM impregnated with PRP lysate, the greatest growth-stimulating effect was observed on the 1st day.
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##article.viewOnOriginalSite##About the authors
Dmitry A. Bozhenko
Helmholtz National Medical Research Center for Eye Diseases
Email: panacelium@gmail.com
Postgraduate Student, Ophthalmologist
Russian Federation, MoscowEkaterina V. Chentsova
Helmholtz National Medical Research Center for Eye Diseases
Email: chentsova27@yandex.ru
ORCID iD: 0000-0002-8394-1038
SPIN-code: 8191-8338
Dr. Sci. (Med.), Professor, Head of the traumatology and reconstructive surgery Department
Russian Federation, MoscowNatalia V. Borovkova
N.V. Sklifosovsky Research Institute for Emergency Medicine
Email: borovkovanv@yandex.ru
ORCID iD: 0000-0002-8897-7523
SPIN-code: 9339-2800
Dr. Sci. (Med.), Head of the Biotechnology and Transfusiology Department
Russian Federation, MoscowIvan N. Ponomarev
N.V. Sklifosovsky Research Institute for Emergency Medicine
Email: rzam@yandex.ru
ORCID iD: 0000-0002-2523-6939
SPIN-code: 4705-9314
Cand. Sci. (Med.), Senior Research Associate
Russian Federation, MoscowMaya V. Storozheva
N.V. Sklifosovsky Research Institute for Emergency Medicine
Email: Mayya.storozheva@yandex.ru
ORCID iD: 0000-0003-1927-2404
SPIN-code: 7789-3277
Researcher
Russian Federation, MoscowMaksim S. Makarov
N.V. Sklifosovsky Research Institute for Emergency Medicine
Email: mcsimmc@yandex.ru
SPIN-code: 3543-5800
Cand. Sci. (Med.), Senior Research Associate
Russian Federation, MoscowPavel V. Makarov
Helmholtz National Medical Research Center for Eye Diseases
Author for correspondence.
Email: makarovpavel61@mail.ru
Dr. Sci. (Med.), Leading Research Associate
Russian Federation, MoscowReferences
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