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Post a Comment Modification of emulsified fat (nanofat) obtaining procedures
- Authors: Khramtsova N.I.1, Plaksin S.A.1, Gulyaeva N.I.1, Sotskov A.Y.1, Ponomarev D.N.1
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Affiliations:
- E.A. Vagner Perm State Medical University
- Issue: Vol 39, No 1 (2022)
- Pages: 66-73
- Section: Methods of diagnosis and technologies
- URL: https://journals.rcsi.science/PMJ/article/view/81121
- DOI: https://doi.org/10.17816/pmj39166-73
- ID: 81121
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Abstract
Objective. To modify the procedure by reducing the number of filters and passages through them.
Materials and Methods. 16 samples of fat, aspirated by a syringe from the abdominal region were examined. Fat filtration was carried out through anaerobic fat transfers with an inner diameter of 1.4 mm and 1.2 mm, as well as an emulsifying filter (nanofat filter). The content of destroyed adipocytes and fibroblast-like cells was assessed.
Results. The 1.2 mm filter with 10 passages protocol provides determination of the minimal number of adipocytes while maintaining fibroblast-like cells. While filtrating through the 1.4 mm transfer, and then immediately through the “nanofat filter”, numerous connective tissue fibers with fibroblast-like cells are identified. When filtrating an adipose tissue using the 1.4 mm transfer 10 times, then – using “nanofat filter” 5 times, there was obtained a homogeneous fat emulsion with a high content of morphologically intact adipocytes, the particle diameter of which allows injecting with a thin needle syringe.
Conclusions. To obtain “nanofat”, it is possible to optimize the lipograft filtration protocol proposed by P. Tonnard (2013), depending on the purpose of using the obtained product.
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##article.viewOnOriginalSite##About the authors
N. I. Khramtsova
E.A. Vagner Perm State Medical University
Email: renelve@gmail.com
Candidate of Medical Sciences, Associate Professor, Department of Public Health and Healthcare № 1
Russian Federation, PermS. A. Plaksin
E.A. Vagner Perm State Medical University
Author for correspondence.
Email: splaksin@mail.ru
MD, PhD, Professor, Department of Surgery with Course of Cardiovascular Surgery and Invasive Cardiology
Russian Federation, PermN. I. Gulyaeva
E.A. Vagner Perm State Medical University
Email: bizon55@mail.ru
Candidate of Medical Sciences, Associate Professor, Department of Histology, Embryology and Cytology
Russian Federation, PermA. Yu. Sotskov
E.A. Vagner Perm State Medical University
Email: Sozkov1998a@mail.ru
sixth year student
Russian Federation, PermD. N. Ponomarev
E.A. Vagner Perm State Medical University
Email: danilpon07@gmail.com
sixth year student
Russian Federation, PermReferences
- Tonnard P., Verpaele A., Peeters G., Hamdi M., Cornelissen M., Declercq H. Nanofat grafting: basic research and clinical applications. Plast Reconstr Surg 2013; 132 (4): 1017-1026.
- Sesé B., Javier M., Ortega B., Matas-Palau A., Llull R. Nanofat Cell Aggregates: A Nearly Constitutive Stromal Cell Inoculum for Regenerative Site-Specific Therapies. Plast Reconstr Surg 2019; 144 (5): 1079-1088.
- Vasilyev V., Vasilyev S., Vazhenin A., Teryushkova Z., Vasilyev Y., Vasilyev I., Semyonova A., Dimov G., Lomakin E. An Algorithm for Treatment of Radiation-Induced Soft Tissue Damage with Products Based on Autologous Adipose Tissue. Plast Reconstr Surg Glob Open 2018; 6 (9): 155-156.
- Pascucci L., Mercati F., Marini C., Ceccarelli P., Dall'Aglio C., Pedini V., Gargiulo A.M. Ultrastructural morphology of equine adipose-derived mesenchymal stem cells. Histol Histopathol 2010; 25 (10): 1277-1285.
- Khramtsova N., Plaksin S., Sotskov A., Ponomarev D. Anaerobic fat transfers and emulsifiers for autologous fat grafting. Medical Measurements and Applications, MeMeA. Symposium Proceedings (SCOPUS) 2020.
- Vasilyev V., Vasilyev S., Teryushkova Z., Vasilyev Y., Vasilyev I., Eremin I., Dimov G., Lomakin E. Mechanisms of biological action various products based on Autologous lipoaspirate and the possibilities of their clinical application. Materials of the IV National congress on regenerative medicine 2019; 6 (9): 50 (in Russian).
- Osinga R., Menzi N.R., Tchang L., Caviezel D., Kalbermatten D.F., Martin I., Schaefer D.J., Scherberich A., Largo R.D. Effects of intersyringe processing on adipose tissue and its cellular components: implications in autologous fat grafting. Plastic and reconstructive surgery 2015; 135 (6): 1618-1628.
- Chen X., Hong S., Hong F., Yang B., Tong C., Zhang J. Mechanical emulsification of lipoaspirate by different Luer-Lok connector changes the viability of adipose derived stem cells in Nanofat. J Plast Surg Hand Surg 2020; 54 (6): 344-351.
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