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Post a Comment Prolongation of viability of isolated skin explants by exposing them to their own transformed physical fields: experimental study
- Authors: Nikityuk I.E.1, Afonichev K.A.1, Nikitin M.S.1, Kubasov V.A.2, Petrash V.V.3
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Affiliations:
- H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery
- D.V. Efremov Scientific Research Institute of Electrophysical Apparatus (NIIEFA)
- Private Institution “Educational Organization of Higher Education” Medical University “REAV-IZ”
- Issue: Vol 37, No 6 (2020)
- Pages: 131-142
- Section: Clinical case
- URL: https://journals.rcsi.science/PMJ/article/view/59595
- DOI: https://doi.org/10.17816/pmj376131-142
- ID: 59595
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Abstract
Objective. To study the effect of materials with optical-electrical properties on the survival of full-layer skin explants when they are isolated from the body with completely stopped trophic activity.
Material and methods. In rabbits of both sexes at the age of 5-6 months, full-layer skin flaps were excised from the back area, which were dissected into fragments-explants measuring 1.0×1.0 cm. All samples were thermostated for 2 days at 37 °C near transducers represented by aluminum foil sheets and silicon single crystals. In the first series of experiments, the explants were directly in contact with the transducers; in the second and third series, screens made of light-proof black paper and thin glass were installed between the explants and the transducers, respectively. In each series, the experiments were repeated 5 times with each transducer. After thermostating, the explant viability index was evaluated by histological method in points.
Results. In the first and second series of experiments, the highest survival rate of explants with a viability index from 3.6 to 3.8 points with a norm of 4.0 points) was revealed depending on the type of transducer. In series III, when screening silicon crystals with thin glass, the explant survival rate decreased slightly to 3.3 points. However, the screening of aluminum foil with glass led to a sharp decrease in the viability of explants to 0.2 points.
Conclusions. A long-term viability of skin explants can be explained only by the effect of their own physical fields on them produced by the biostructures of explants and transformed by interaction with nearby materials possessing optical and electrical properties.
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##article.viewOnOriginalSite##About the authors
I. E. Nikityuk
H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery
Author for correspondence.
Email: femtotech@mail.ru
Candidate of Medical Sciences, Leading Researcher, Laboratory of Physiological and Biomechanical Researches
Russian Federation, Saint PetersburgK. A. Afonichev
H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery
Email: femtotech@mail.ru
MD, PhD, Head of Unit of Trauma and Rheumatoid Arthritis Consequences
Russian Federation, Saint PetersburgM. S. Nikitin
H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery
Email: femtotech@mail.ru
traumatologist-orthopedist, Unit of Trauma and Rheumatoid Arthritis Consequences
Russian Federation, Saint PetersburgV. A. Kubasov
D.V. Efremov Scientific Research Institute of Electrophysical Apparatus (NIIEFA)
Email: femtotech@mail.ru
Candidate of Physical and Mathematical Sciences, Senior Researcher, Scientific Research Laboratory KL-3 BI-3
Russian Federation, Saint PetersburgV. V. Petrash
Private Institution “Educational Organization of Higher Education” Medical University “REAV-IZ”
Email: femtotech@mail.ru
Doctor of Biological Sciences, Professor, Department of Medico-Biological Disciplines
Russian Federation, Saint PetersburgReferences
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