DYNAMICS OF MICROCIRCULATION INDEX CHANGES IN ISCHEMIC SPINAL MARROW EXPOSED TO RECOMBINANT ERYTHROPOIETIN AND LASER EMISSION (experimental study)

Aim. To study the dynamics of microcirculation index in the focus of experimental spinal marrow ischemia in rats exposed to recombinant erythropoietin and laser emission. Materials and methods. The work was carried out on 80 sexually mature rats, divided into four test series - 20 animals each. In animals of series one, the control group, the spinal marrow ischemia was modeled. In series two, animals were intraperitoneally introduced 1000 ME of recombinant erythropoietin in 3, 24 and 48 hours. Animals of series three after the operation were exposed to skin irradiation with diode laser in the zone of ischemia. In test series four, laser emission of the ischemic focus was combined with introduction of recombinant erythropoietin. Assessment of microcirculation index was performed in animals of all the series during each period of the test using the unit LAKK-01 (Russian Federation) with subsequent mathematical and statistical processing by means of the program “Lasma” (Russian Federation). Results. The study results reliably indicate that recombinant erythropoietin possesses an antiapoptotic and antihypoxic effect, stimulates angio- and neurogenesis, but when exposed to laser emission of infrared range it promotes strengthening of microcirculation in the focus of ischemia. Conclusions. Combined effect of recombinant erythropoietin and laser emission in the zone of ischemic lesion of the spinal marrow significantly raises the local blood flow. It is accompanied by an early regress of neurological disorders and activation of behavior in animals.

Spinal marrow, ischemia, recombinant erythropoietin, laser emission, microcirculation index