Experimental studies of effectiveness of a new mineral complex for regeneration of epithelial tissues and skin

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Abstract

Objective. To study the regenerative activity of a mineral complex containing ions of cerium, manganese, zinc and silver in model experiments of the planar skin wounds and acute bronchitis in laboratory animals for the development of new techniques of surgical and pulmonological diseases treatment.

Materials and methods. An experimental study of the regenerative activity of a mineral complex containing ions of cerium, manganese, zinc and silver was carried out on models of the planar skin wounds and acute bronchitis in rats.

Results. The results of histological examination of tissues showed the advantage of the mineral complex over the control groups of animals. The anti-inflammatory and regenerative activity of the mineral complex was established in a model of acute bronchitis and regenerative activity in a model of a planar skin wound.

Conclusions. The effectiveness of the mineral complex depended on the concentration of cerium ions and was manifested at a dose of 0.003 μg/kg of animal weight.

About the authors

V. Yu. Mishlanov

E.A. Vagner Perm State Medical University

Author for correspondence.
Email: permmed@hotmail.com

MD, PhD, Professor, Correspondent Member of RAS, Head of the Department of Propaedeutics of Internal Diseases №1

Russian Federation, Perm

V. А. Chereshnev

Institute of Immunology and Physiology, Ural Branch of RAS

Email: permmed@hotmail.com

MD, PhD, Professor, Academician of RAS, Chief Researcher

Russian Federation, Perm

G. P. Vdovina

E.A. Vagner Perm State Medical University

Email: permmed@hotmail.com

PhD, Professor, Head of the Department of Pharmacology

Russian Federation, Perm

А. А. Burlutskaya

E.A. Vagner Perm State Medical University

Email: permmed@hotmail.com

researcher, Department of Pre-clinical Studies, Central Research Laboratory

Russian Federation, Perm

А. L. Zuev

Perm National Research Polytechnic University; Institute of Continuous Media Mechanics of Perm Federal Research Center of Ural Branch of RAS

Email: permmed@hotmail.com

Doctor of Physical and Mathematical Sciences, Associate Professor, Professor of the Department of Applied Physics, Senior Researcher

Russian Federation, Perm; Perm

E. P. Koshurnikova

E.A. Vagner Perm State Medical University

Email: permmed@hotmail.com

Candidate of Medical Sciences, Associate Professor, Department of Propaedeutics of Internal Diseases №1

Russian Federation, Perm

V. V. Emelkina

E.A. Vagner Perm State Medical University

Email: permmed@hotmail.com

Assistant, Department of Propaedeutics of Internal Diseases №1

Russian Federation, Perm

V. А. Samartsev

E.A. Vagner Perm State Medical University

Email: permmed@hotmail.com

MD, PhD, Professor, Head of the Department of General Surgery

Russian Federation, Perm

V. А. Gavrilov

E.A. Vagner Perm State Medical University

Email: permmed@hotmail.com

Candidate of Medical Sciences, Associate Professor, Department of General Surgery

Russian Federation, Perm

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2. Fig. 1. Dynamics of skin wounds in the experimental and control groups that did not receive treatment

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3. Fig. 2. Comparative histological data in the experimental and control groups: a – control data. Granulation tissue. Leukocyte infiltration. Hematoxylin and eosin staining. Uv. х 400; b – tested mineral complex. Granulation tissue. Cell layer with fibroblasts. Hematoxylin and eosin staining. Uv. х 400

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4. Fig. 3. Comparative results of histological examination in the group receiving the tested mineral complex and control observations: a – structure of the wall of a medium-sized bronchus after inhalation of a 1% formaldehyde solution. Hematoxylin and eosin staining. Uv. х 400; b – structure of the wall of a medium-caliber bronchus after inhalation of a cerium preparation at a dose of 0.003 mcg/kg. Hematoxylin and eosin staining. Uv. х 400; c – structure of the wall of a medium-sized bronchus after inhalation of a 0.9% NaCl solution. Hematoxylin and eosin staining. Uv. х 400

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