Induction of immune response caused by G-01 solution in laboratory animals with transplanted melanoma (B-16)

Objective. To study the properties of the immune response with G-01 solution at the example of melanoma (B-16).

Material and methods. Experiments were conducted on the offspring of heterosexual BALB/c aged 8 weeks. Groups of offspring were not separated by sex. In the work, a 20 % solution of G-01 was used, administered subcutaneously 0.5 ml 2 times a day for 2 days with the presence of an adjutant (aluminum hydroxide). In the first experiment, the solution was used as a prophylactic drug, in the second – as a therapeutic one, which was used on the 6th day after the transplantation of melanoma (B-16).

Results. In the first experiment, one month after the applied treatment scheme (prophylactic) with G-01 solution, an attempt was made to transplant the melanoma (B-16) subcutaneously to 10 mice of the main group. The control group consisted of 6 mice. After 3 weeks, an increase in the neoplasm of the right axillary region was detected in 2 mice of the main group. The neoplasm regressed during the next 3 weeks. In the control group, melanoma transferred in 5 out of 6 mice, with subsequent development of the disease and fatal outcome on average on the 60th day of the disease. In the second experiment, two groups (10 animals each) were analyzed: control and main. The tumor transferred in 100 % of cases in both groups. The injected solution on the 6th day after the transplantation of melanoma (B-16) did not affect the growth of the tumor. Mice died in both groups. The average duration of life in both groups was 36 and 38 days, respectively. There were no statistically significant differences in the groups.

Conclusions. The prospect of using G-01 solution in medicine as a preventive agent of natural origin was established. This method of inducing an immune response with G-01 solution can be used to prevent the development of a solid tumor of melanoma B-16. The subcutaneously injected 20 % solution of G-01 – 0.5 ml 2 times a day for 2 days – has no effect on melanoma on the 6th day after the transplantation.