EFFECT OF PEGYLATED GRAPHENE OXIDE NANOPARTICLES ON THE METABOLISM OF JURKAT CELLS

V. P. Timganova; Тимганова В. П.; V. V. Vlasova; Власова В. В.; M. S. Bochkova; Бочкова М. С.; K. Yu. Shardina; Шардина К. Ю.; S. V. Uzhviyuk; Ужвиюк С. В.; P. V. Khramtsov; Храмцов П. В.; M. B. Rayev; Раев М. Б.; S. A. Zamorina; Заморина С. А.

doi:10.31857/S2686738923600292

EFFECT OF PEGYLATED GRAPHENE OXIDE NANOPARTICLES ON THE METABOLISM OF JURKAT CELLS

Авторлар: Timganova V.¹, Vlasova V.¹, Bochkova M.¹^,2, Shardina K.¹, Uzhviyuk S.¹, Khramtsov P.¹^,2, Rayev M.¹^,2, Zamorina S.¹^,2
Мекемелер:
1. Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences
2. Perm State National Research University
Шығарылым: Том 512, № 1 (2023)
Беттер: 470-473
Бөлім: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/140859
DOI: https://doi.org/10.31857/S2686738923600292
EDN: https://elibrary.ru/TASLVY
ID: 140859

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Аннотация

The effect of graphene oxide (GO) nanoparticles of 100–200 nm size coated with linear (LP-GO) and branched (BP-GO) polyethylene glycol at concentrations of 5 and 25 μg/mL on the metabolism of Jurkat tumor cells was studied. It was found that LP-GO nanoparticles at a concentration of 25 μg/mL can enhance basal glycolysis of Jurkat T lymphocyte tumor cell line cells, while LP-GO and BP-GO at the same concentration can reduce the indicators of compensatory glycolysis. Despite this, GO nanoparticles coated with linear and branched PEG at a concentration of 5 μg/mL do not have pronounced effects on oxidative phosphorylation and glycolysis of Jurkat cells and could therefore be safe for activated T cells.

Негізгі сөздер

PEGylated graphene oxide nanoparticles, Jurkat cell line, metabolism, mitochondrial respiration, aerobic glycolysis

Әдебиет тізімі

Priyadarsini S., Mohanty S., Mukherjee S., et al. Graphene and graphene oxide as nanomaterials for medicine and biology application // Journal of Nanostructure in Chemistry. 2018. № 8. P. 123–137.
Zare P., Aleemardani M., Seifalian A., et al. Graphene Oxide: Opportunities and Challenges in Biomedicine // Nanomaterials (Basel). 2021. V. 11. № 5. 1083.
Zhang L., Xia J., Zhao Q., et al. Functional Graphene Oxide as a Nanocarrier for Controlled Loading and Targeted Delivery of Mixed Anticancer Drugs // Small. 2010. № 6. P. 537–544.
Vander Heiden M.G., Cantley L.C., Thompson C.B. Understanding the Warburg effect: the metabolic requirements of cell proliferation // Science. 2009. V. 324. № 5930. P. 1029–1033.
Cantor J.R., Sabatini D.M. Cancer cell metabolism: one hallmark, many faces // Cancer Discovery. 2012. V. 2. № 10. P. 881–898.
Шейбак В.М., Павлюковец А.Ю. Биохимическая гетерогенность Т-лимфоцитов // Вестник Витебского государственного медицинского университета. 2018. Т. 17. № 6. С. 7–17.
Montano M. Translational Biology in Medicine. Woodhead Publishing. Cambridge. 2015.
Khramtsov P.V., Bochkova M.S., Timganova V.P. et al. Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood // Nanomaterials. 2021. V. 12. e:126.
Romero N., Swain P., Neilson A., et al. Improving Quantification of Cellular Glycolytic Rate Using Agilent Seahorse XF Technology (White Paper) Agilent Technologies, Inc (2017) (5991-7894EN).
Zamorina S.A., Khramtsov P.V., Rayev M.B. et al. Graphene Oxide Nanoparticels Interaction with Jurkat Cell Line in Cell-IQ System. Doklady Biochemistry and Biophysics. 2021. V. 501. P. 438–443.
Chang X., Liu X., Wang H., et al. Glycolysis in the progression of pancreatic cancer // American Journal of Cancer Research. 2022. V. 12. № 2. P. 861–872.