THE NCoR CO-REPRESSOR INTERACTS WITH THE KAISO TRANSCRIPTION FACTOR THROUGH A MECHANISM DIFFERENT FROM THAT OF BCL6 INTERACTION

K. I. Balagurov; Балагуров К. И.; P. G. Georgiev; Георгиев П. Г.; A. N. Bonchuk; Бончук А. Н.

doi:10.31857/S2686738922600777

THE NCoR CO-REPRESSOR INTERACTS WITH THE KAISO TRANSCRIPTION FACTOR THROUGH A MECHANISM DIFFERENT FROM THAT OF BCL6 INTERACTION

Authors: Balagurov K.I.¹, Georgiev P.G.¹, Bonchuk A.N.¹
Affiliations:
1. Institute of Gene Biology, Russian Academy of Sciences
Issue: Vol 508, No 1 (2023)
Pages: 91-94
Section: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/135684
DOI: https://doi.org/10.31857/S2686738922600777
EDN: https://elibrary.ru/MPYLIL
ID: 135684

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Abstract

The vertebrate transcription factor Kaiso binds specifically to methylated DNA sequences using C2H2-type zinc fingers. In addition to C2H2-domains, the BTB/POZ domain, which forms homodimers, is located at the N-terminus of Kaiso. Kaiso, like several other well-studied BTB/POZ proteins, including BCL6, interacts with the NCoR (nuclear co-repressor) protein, which determines the landing of transcriptional repressive complexes on chromatin. Using the yeast two-hybrid system, we have shown that the N-terminal domain of NCoR interacts with the C-terminal zinc fingers of Kaiso, and not with its BTB/POZ domain, as previously assumed. The results obtained demonstrate that NCoR interacts with various transcription factor domains, which can increase the efficiency of attracting NCoR-dependent repressor complexes to regulatory regions of the genome.

Keywords

co-repressors, zinc-finger proteins, C2H2-domains, transcription factors, transcriptional repression

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