Email this article SUBPOPULATION HETEROGENEITY OF NK CELLS DURING THE GENETIC MODIFICATION FOR SUBSEQUENT USE IN TARGETED THERAPY
- Authors: Streltsova M.A.1, Deev S.M.1,2, Sapozhnikov A.M.1, Grechikhina M.V.1, Vavilova J.D.1, Velichinskii R.A.1, Alekseeva N.A.1, Palamarchuk A.I.1, Ustiuzhanina M.O.1,3, Boyko A.A.1, Kovalenko E.I.1
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Affiliations:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- I.M. Sechenov First Moscow State Medical University
- Skolkovo Institute of Science and Technology
- Issue: Vol 508, No 1 (2023)
- Pages: 41-44
- Section: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/135672
- DOI: https://doi.org/10.31857/S2686738922700068
- EDN: https://elibrary.ru/MVBLMI
- ID: 135672
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Abstract
Obtaining genetically engineered NK cells is one of the developing areas of immunotherapy. In this work, we analyzed the subset heterogeneity of NK cells subjected to retroviral transduction, taking into account the content of adaptive NK cell precursors. It has been shown that subpopulations of KIR2DL2/DL3+, as well as CD57–KIR2DL2/DL3+NKG2C+, can be modified with greater efficiency than the corresponding subpopulations that do not carry the KIR2DL2/DL3 and NKG2C markers. After genetic modification, the CD57–KIR2DL2/DL3+NKG2C+ cells began to express CD57 de novo, acquiring the adaptive NK cell phenotype.
About the authors
M. A. Streltsova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
S. M. Deev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University
Email: lenkovalen@mail.ru
Russian Federation, Moscow; Russian Federation, Moscow
A. M. Sapozhnikov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
M. V. Grechikhina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
J. D. Vavilova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
R. A. Velichinskii
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
N. A. Alekseeva
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
A. I. Palamarchuk
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
M. O. Ustiuzhanina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Skolkovo Institute of Science and Technology
Email: lenkovalen@mail.ru
Russian Federation, Moscow; Russian Federation, Moscow
A. A. Boyko
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: lenkovalen@mail.ru
Russian Federation, Moscow
E. I. Kovalenko
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: lenkovalen@mail.ru
Russian Federation, Moscow
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