Resistance of rice Oryza sativa L.. doubled haploids to lodging for the far eastern breeding

The phenotypic variability of rice Oryza sativa L. androgenic doubled haploids lines (DH), intended for lodging resistance breeding, was studied. The lines used were obtained from F2 hybrids of the combinations Kitaets×(VNIIR23×Kenzo) - K×V×K (plants No. 26 and No. 28) and Don 4237× (Szorvasii 70× Heilunjiang) - D×Z×H (plant No. 8). Variety Primorsky 29 served as the standard. In 2020, DH lines and parental forms were grown under the conditions of a culture room in plastic cups filled with soil (temperature 25 °C, illumination 5000 lux, day 16 hours). In 2021 the seed offspring of the previous year were sown on the growing area in vessels 360×60 cm in size, 0.65 m3 in volume, filled with field soil. Each sample was sown in two rows, 25 plants per row. According to the ANOVA results, the DH lines and parental forms differed from each other in all characteristics in both years (p<0.018). The panicle grain mass rises with an increasing culm diameter, which is appeared in the average correlation of these features (r=0.63, p<0.05). The use of haploid technology anther culture in vitro made it possible to obtain the DH lines exceeded the parental forms by 0.67-1.24 mm and the control variety Primorsky 29 by 0.06-0.61 mm in diameter and straw strength index by 25-50%. At the same time, the productivity of the panicle remained at the control level (0.9-1.2 g) in most of the studied lines. One line exceeded the number of grains by 11.1-16.4 pcs. and the mass of grain of the main panicle per 0.5-0.7 g parental forms and control variety. Early maturity was transferred from one of the parental forms to the DH lines. A number of doubled haploids lines have been created for rice O. sativa breeding for lodging resistance, which is necessary for the Far Eastern rice cultivation.

Oryza sativa, doubled haploids, lodging resistance