Email this article Assessment the Kalmyk cattle breed genetic diversity in the Kalmykia republic based on genome-wide analysis
- Authors: Gorlov I.F1, Slozhenkina M.I1, Fedorov Y.N2, Anisimova E.Y.1, Karpenko E.V1, Badmaeva K.E1, Ubushieva V.S1
-
Affiliations:
- Volga region Research Institute of manufacture and processing of meat-and-milk production
- All-Russian Research and Technological Institute of Biological industry
- Issue: No 6 (2023)
- Pages: 80-83
- Section: Articles
- URL: https://journals.rcsi.science/2500-2082/article/view/233585
- DOI: https://doi.org/10.31857/2500-2082/2023/6/80-83
- EDN: https://elibrary.ru/WSFHLX
- ID: 233585
Cite item
Open Access
Access granted
Subscription Access
Abstract
In the article results of the biodiversity evaluation of the indigenous breed resources of cattle of the Republic of Kalmykia by whole-genome genotyping are presented. The objects of research were samples of biomaterial obtained from two herds of Kalmyk cattle raised in the Republic of Kalmykia (n = 20). Other samples for population-genetic studies were taken from L.K. Ernst FRC (Podolsk, Russia). Modern molecular-genetics and bioinformatics methods were used in this research. As a result, DNA was isolated and comparative study was carried out. The obtained DNA samples are suitable for carrying out whole-genome SNP studies using Bovine SNP HD BeadChip according to their qualitative and quantitative characteristics. The concentration of double-stranded DNA in the studied samples varied up 16 to 80 ng/µl, the ratio of OD260/OD280 varied up 1.77 to 2.12. The efficiency of genotyping (call rate) varied up 99.72 to 99.84%. The novelty of this research is the genetic diversity of two populations of Kalmyk cattle raised in the Republic of Kalmykia was established at the first time using high-density DNA chips and population-genetic estimation was also carried out for comparison with three breeds of Turano-Mongolian origin. Kalmyk breed have shown the highest level of genetic diversity compared to other breeds of Turano-Mongolian origin. This fact may be a consequence of the use of free mating in the breeding , as well as a relatively high number of Kalmyk cattle compared to other breeds. The minimum level of genetic diversity established in the Yakut breed is a consequence of the small number of livestock. The results obtained will be useful in the development of breeding and conservation programs of the Kalmyk cattle.
About the authors
I. F Gorlov
Volga region Research Institute of manufacture and processing of meat-and-milk production
Email: niimmp@mail.ru
M. I Slozhenkina
Volga region Research Institute of manufacture and processing of meat-and-milk production
Yu. N Fedorov
All-Russian Research and Technological Institute of Biological industry
E. Yu Anisimova
Volga region Research Institute of manufacture and processing of meat-and-milk production
E. V Karpenko
Volga region Research Institute of manufacture and processing of meat-and-milk production
K. E Badmaeva
Volga region Research Institute of manufacture and processing of meat-and-milk production
V. S Ubushieva
Volga region Research Institute of manufacture and processing of meat-and-milk production
References
- Генджиева О.Б., Сулимова Г.Е. Изучение генетического разнообразия калмыцкого скота с использованием ISSR-фингерпринтинга // Зоотехния. 2009. № 3. С. 4-5.
- Зиновьева Н.А., Доцев А.В., Сермягин А.А. и др. Изучение генетического разнообразия и популяционной структуры российских пород крупного рогатого скота с использованием полногеномного анализа SNP // Сельскохозяйственная биология. 2016. Т. 51. № 6. С. 788-800.
- Ковальчук А.М. Генетическое разнообразие казахской белоголовой породы крупного рогатого скота // Известия Оренбургского государственного аграрного университета. 2021. № 6 (92). С. 283-289.
- Моисейкина Л.Г., Каюмов Ф.Г., Чимидова Н.В. и др. Аллелофонд крупного рогатого скота калмыцкой породы разных зональных типов // Сборник научных трудов SWorld. 2013. Т. 49. № 4. С. 6-12.
- Улимбашев М.Б., Гостева Е.Р., Краснова О.А. и др. Состояние мясных породных ресурсов крупного рогатого скота России (обзор) // Генетика и разведение животных. 2023. № 1. С. 52-64.
- Федоров В.Х., Приступа В.Н., Бабкин О.А., Торосян Д.С. Совершенствование скота калмыцкой породы : монография / под общей редакцией О.А. Бабкина. Персиановский: Донской ГАУ, 2021. 168 с.
- Чимидова Н.В., Моисейкина Л.Г., Болдырев Б.А., Черунова И.В. Генетическое сходство калмыцкого скота племенных хозяйств республики Калмыкия // Современные проблемы науки и образования. 2013. № 2. С. 486.
- Чимидова Н.В., Моисейкина Л.Г., Убушиева А.В. и др. Изменения генофонда скота калмыцкой породы // Животноводство и кормопроизводство. 2020. Т. 103. № 4. С. 65-73.
- Чимидова Н.В., Моисейкина Л.Г., Убушиева А.В. и др. Генетическое сходство калмыцкого скота в племенных хозяйствах республики Калмыкия // Аграрно-пищевые инновации. 2021. № 4 (16). С. 28-35.
- Юдин Н.С., Ларкин Д.М. Происхождение, селекция и адаптация российских пород крупного рогатого скота по данным полногеномных исследований // Вавиловский журнал генетики и селекции. 2019. Т. 23. № 5. С. 559-568.
- Abdelmanova A.S., Kharzinova V.R., Volkova V.V. et al. Comparative Study of the Genetic Diversity of Local Steppe Cattle Breeds from Russia, Kazakhstan and Kyrgyzstan by Microsatellite Analysis of Museum and Modern Samples // Diversity. 2021. Vol. 13. № 8. Article 351. https://doi.org/10.3390/d13080351.
- Chimidova N.V., Moiseikina L.G., Ubushieva A.V. et al. Genetic structure of population of the Kalmyk breed cattle // E3S WEB OF CONFERENCES. XV International Scientific Conference on Precision Agriculture and Agricultural Machinery Industry “State and Prospects for the Development of Agribusiness - Interagromash 2022”. Rostov-on-Don, 2022.
- Sermyagin A.A., Dotsev A.V., Gladyr E.A. et al. Whole-genome SNP analysis elucidates the genetic structure of Russian cattle and its relationship with Eurasian taurine breeds // Genetics Selection Evolution. 2018. Vol. 50. Article 37. https://doi.org/10.1186/s12711-018-0408-8.
