Assessment of naphazoline effect on erythrocyte superoxide dismutase activity in rats under experimental modeling of acute radiation sickness
- Authors: Ivanov V.S.1, Seleznev A.B.2, Raguzin E.V.2, Ivchenko E.V.1, Pechurina T.B.2, Ivanov I.M.2, Glushenko D.D.1, Glushakov R.V.1
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Affiliations:
- Military Medical Academy named after S.M. Kirov
- State Research Scientific Institute of Military Medicine
- Issue: Vol 8, No 1 (2023)
- Pages: 60-65
- Section: Pharmacology
- URL: https://journals.rcsi.science/2500-1388/article/view/131502
- DOI: https://doi.org/10.35693/2500-1388-2023-8-1-60-65
- ID: 131502
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Abstract
Aim – to assess the activity of superoxide dismutase in erythrocytes of rats after a single intramuscular injection of naphazoline in radiofrequency dose and in experimental simulation of acute radiation sickness.
Material and methods. The activity of erythrocytes superoxide dismutase (SOD) after single intramuscular injection of nafazoline in experimental modeling of acute radiation sickness was investigated. The pharmacological properties and mechanisms of radioprotective action of nafazoline were clarified based on the dynamics of activity of erythrocytes SOD in intact and irradiated animals with the dose of 7,4 Gy.
Results. The superoxide dismutase activity was found to increase significantly 60 min after single intramuscular administration of nafazolin at a dose of 5 mg/kg. Under experimental conditions of acute radiation disease modeling, a decrease in superoxide dismutase activity was observed within the first hour after exposure, evidencing the direct involvement of the antioxidant system components in the inactivation of free-radical reaction products.
Conclusion. The radioprotective properties of naphazoline may be due not only to reduction of oxygen delivery to the cells of radiosensitive tissues and inhibition of their metabolism by effect on α2-adreno- and imidazoline receptors, but also by activation of antioxidant system links.
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##article.viewOnOriginalSite##About the authors
Valerii S. Ivanov
Military Medical Academy named after S.M. Kirov
Email: ivanovmed84@mail.ru
ORCID iD: 0000-0002-2643-7767
Senior Resident of the Clinic of Naval Therapy
Russian Federation, Saint-PetersburgAleksei B. Seleznev
State Research Scientific Institute of Military Medicine
Email: alexseleznov@list.ru
ORCID iD: 0000-0002-9278-5698
PhD, Associate Professor, Deputy Head of Research Test Center of Medical and Biological Defense
Russian Federation, Saint-PetersburgEvgenii V. Raguzin
State Research Scientific Institute of Military Medicine
Email: evgeny.raguzin@yandex.ru
ORCID iD: 0000-0002-1707-6912
PhD, Deputy Head of Scientific-Research Testing Department
Russian Federation, Saint-PetersburgEvgenii V. Ivchenko
Military Medical Academy named after S.M. Kirov
Email: 8333535@mail.ru
ORCID iD: 0000-0001-5582-1111
PhD, Associate Professor, Deputy Head of the Military Medical Academy for Scientific Work
Russian Federation, Saint-PetersburgTatyana B. Pechurina
State Research Scientific Institute of Military Medicine
Email: tat79@list.ru
ORCID iD: 0000-0002-8228-2800
PhD, Junior Researcher
Russian Federation, Saint-PetersburgIgor M. Ivanov
State Research Scientific Institute of Military Medicine
Email: igor611ivanov@gmail.com
ORCID iD: 0000-0002-8708-8484
PhD, Head of Research and Development Testing Department
Russian Federation, Saint-PetersburgDaniil D. Glushenko
Military Medical Academy named after S.M. Kirov
Author for correspondence.
Email: glushenko.daniil.d@yandex.ru
ORCID iD: 0000-0001-9425-6565
5th year cadet, 3rd Faculty
Russian Federation, Saint-PetersburgRuslan V. Glushakov
Military Medical Academy named after S.M. Kirov
Email: glushakovruslan@gmail.com
ORCID iD: 0000-0002-0161-5977
PhD, Associate Professor, Head of Research Department (Medical and Biological Research) of Research Center
Russian Federation, Saint-PetersburgReferences
- Grebenyuk AN, Legeza VI. Prospects of the use of radioprotectors for improvement of anti-radiation medicine in the Armed Forces. Russian military medical journal. 2013;7:46-50. (In Russ.). [Гребенюк А.Н., Легеза В.И. Перспективы использования радиопротекторов для повышения эффективности медицинской противорадиационной защиты Вооруженных сил. Военно-медицинский журнал. 2013;7:46-50]. doi: 10.17816/RMMJ74439
- Chapman RA, Leoty C. Which of caffeine’s chemical relatives are able to evoke contractures in mammalian heart? Recent advances in studies on cardiac structure and metabolism. 1975;7:425-430.
- Gladkikh VD, Balandin NV, Basharin VA, et al. Status and prospects for the development of means for the prevention and treatment of radiation injuries. М., 2017. (In Russ.). [Гладких В.Д., Баландин Н.В., Башарин В.А., и др. Состояние и перспективы развития средств профилактики и лечения радиационных поражений. М., 2017].
- Ivanov IM, Ivchenko EV, Yudin MA, et al. Application aspects of medications for inhalation at the prehospital stage of medical evacuation. Bulletin of the Russian Military Medical Academy. 2021;23(4):247-256. (In Russ.). [Иванов И.М., Ивченко Е.В., Юдин М.А., и др. Аспекты применения лекарственных препаратов для ингаляций на догоспитальном этапе медицинской эвакуации. Вестник Российской Военно-медицинской академии. 2021;23(4):247-256]. doi: 10.17816/brmma58989
- Vengerovich NG, Yudin MA, Nikiforov AS, et al. Justification for the choice of antioxidants prospective for administration in the form of aerosols following inhalation of toxic substances. Medline.ru. Russian biomedical journal. 2021;22:35-48. (In Russ.). [Венгерович Н.Г., Юдин М.А., Никифоров А.С., и др. Обоснование выбора антиоксидантов, перспективных для введения в виде аэрозолей при ингаляционном поражении отравляющими веществами. Medline.ru. Российский биомедицинский журнал. 2021;22:35-48].
- Ivanov VS, Seleznev AB, Ivchenko EV, et al. Predictability study of the pharmacodynamic properties of drugs in silico by the example of comparing data on the naphazoline clinical use and the results of computer modeling. Bulletin of the Russian Military Medical Academy. 2020;2(70):171-176. (In Russ.). [Иванов В.С., Селезнев А.Б., Ивченко Е.В., и др. Исследование возможностей прогнозирования фармакодинамических свойств лекарственных препаратов in silico на примере сопоставления данных о клиническом применении нафазолина и результатов компьютерного моделирования. Вестник Российской Военно-медицинской академии. 2020;2(70):171-176].
- Prouillac C, et al. Evaluation, in vitro, of the radioprotection of DNA from γ-rays by naphazoline. Comptes Rendus Biologies. 2006;329(3):196-199.
- Vasin MV. Anti-radiation medicines. M., 2010. (In Russ.). [Васин М.В. Противолучевые лекарственные средства. М., 2010].
- Khurtsilava NN, Pluzhnikov NN, Nakatisa YaA. Oxidative stress and inflammation: pathogenetic partnership. SPb., 2012. (In Russ.). [Хурцилава Н.Н., Плужников Н.Н., Накатиса Я.А. Оксидативный стресс и воспаление: патогенетическое партнерство. СПб., 2012].
- Radioprotective agent. Patent 2144357 RF, MPK7 A61, K31/41 / I.I. Krasilnikov. – No. 96113249/14; dec. 07/04/1996; publ. 01/20/2000. – 2000. – Bul. No. 2] (In Russ.). [Радиозащитное средство. Патент 2144357 РФ, МПК7 А61, К31/41 / И.И. Красильников. – № 96113249/14; заявл. 04.07.1996; опубл. 20.01.2000. – 2000. – Бюл. № 2]. Available at: https://patents.google.com/patent/RU2144357C1/ru
- Drachov IS, Turlakov YuS, Bykov VN, et al. Radioprotective efficacy of intraperitoneal, inhalation or intratracheal administration of naphthizin. Clinical Hospital. 2014;2:25-30. (In Russ.). [Драчев И.С., Турлаков Ю.С., Быков В.Н., и др. Профилактическая радиозащитная эффективность нафтизина при его ингаляционном и интратрахеальном введении. Клиническая больница. 2014;2:25-30].
- Vladimirov VG, Krasilnikov II. On some results and prospects for the development of preventive radiation pharmacology. Reviews of clinical pharmacology and drug therapy. 2011;9(1):44-50. (In Russ.). [Владимиров В.Г., Красильников И.И. О некоторых итогах и перспективах развития профилактической радиационной фармакологии. Обзоры по клинической фармакологии и лекарственной терапии. 2011;9(1):44-50].
- Onasanwo SA, Edesiri TP, Adebimpe-John EO. Alpha-2 adrenergic and Cyclo-Oxygenase Mechanisms in Lipopolysaccharide-induced neuropathic pain in Rats. Archives of Basic and Applied Medicine. 2016;4:87-94.
- Abilev SK, Sviridova DA, Grebenyuk AN, et al. Study of the prooxidant and antioxidant activity of anti-radiation agents with lux-biosensors. Radiation biology. Radioecology. 2019;59(5):475-487. (In Russ.). [Абилев С.К., Свиридова Д.А., Гребенюк А.Н., и др. Изучение про- и антиоксидантной активностей противолучевых средств с помощью lux-биосенсоров. Радиационная биология. Радиоэкология. 2019;59(5):475-487]. doi: 10.1134/S0869803119040039
- Mourret A, Agnius C, Rinaldi R. Etude de l'efficacite de trois heterocyles azotes radioprotecteurs sur des souris c3h irradiees au cobalt 60. Comptes rendus de l'Académie des Sciences. 1972;275(14):1575-1578.
- Shulenin KS, Cherkashin DV, Chumakov AV, et al. Schientific and historical aspects of medical care assistance for submarines caught in radioactive accidents. Marine medicine. 2018;4(1):61-68. (In Russ.). [Шуленин К.С., Черкашин Д.В., Чумаков А.В., и др. Научно-исторические аспекты оказания медицинской помощи подводникам при радиационных авариях. Морская медицина. 2018;4(1):61-68]. doi: 10.22328/2413-5747-2018-4-1-61-68
- Shakhmardanova SA, Gulevskya ON, Seletskya VV, et al. Antioxidants: classification, pharmacological properties the use in the practice of medicine. 2016;3:3-15. Zhurnal fundamental'noi meditsiny i biologii. 2016;3:3-15. (In Russ.). [Шахмарданова С.А., Гулевская О.Н., Селецкая В.В., и др. Антиоксиданты: классификация, фармакотерапевтические свойства, использование в практической медицине. Журнал фундаментальной медицины и биологии. 2016;3:3-15].
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