RAPID URINE SCREENING FOR BACTERIURIA IN CHILDREN USING MICROBIOLOGY ANALYZER, COMBINING PHOTOMETRIC AND COHERENT FLUCTUATION NEPHELOMETRIC METHODS
- Authors: Gur’ev A.S.1, Kuznetsova O.Y.2, Pyasetskaya M.F.3, Smirnova I.A.3, Belyaeva N.A.3, Verbov V.N.2, Volkov A.Y.4
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Affiliations:
- Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation St. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian Federation
- St. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian Federation
- N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg, Russian Federation
- Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation Medtechnopark LTD, Moscow, Russian Federation
- Issue: Vol 6, No 4 (2016)
- Pages: 395-398
- Section: METHODS
- URL: https://journals.rcsi.science/2220-7619/article/view/118544
- DOI: https://doi.org/10.15789/2220-7619-2016-4-395-398
- ID: 118544
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Abstract
One of the most widespread microbiological analyses in clinical practice is urine culture, and up to 80% of urine samples turn out to be negative. In this study we demonstrate applicability of coherent fluctuating nephelometry (CFN) method for rapid urine screening and negative samples identification for the purpose of reducing the workload of microbiological laboratory. 205 urine samples were tested by conventional culture method (CCM) and using CFN-analyzer. The agreement between CCM and CFN was 87.8%. Compared to CCM, CFN demonstrated sensitivity — 94.5%, specificity — 85.3%, negative predictive value — 97.7%, positive predictive value — 70.3%. In this study CFN-analyzer allowed to identify 85.3% of negative urine samples (63.9% of all tested samples) within 4 hours, and 94.5% of positive samples were retained for later CCM. Moreover none of samples with count ≥ 104 CFU/ml was omitted.
About the authors
A. S. Gur’ev
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian FederationSt. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian Federation
Author for correspondence.
Email: coherneph@mail.ru
Master (Physics and Mathematics), Researcher, Laboratory of Nanotechnology in Medicine, Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine; Researcher, Medtechnopark LTD, Moscow, Russian Federation Russian Federation
O. Yu. Kuznetsova
St. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian Federation
Email: coherneph@mail.ru
Master of Science in Biotechnology, Researcher, Laboratory Of Nanotechnology, Department Of New Technology Russian Federation
M. F. Pyasetskaya
N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg, Russian Federation
Email: coherneph@mail.ru
Bacteriologist, Clinical Diagnostic Laboratory Russian Federation
I. A. Smirnova
N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg, Russian Federation
Email: coherneph@mail.ru
Bacteriologist, Clinical Diagnostic Laboratory Russian Federation
N. A. Belyaeva
N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg, Russian Federation
Email: coherneph@mail.ru
Bacteriologist, Clinical Diagnostic Laboratory Russian Federation
V. N. Verbov
St. PetersburgPasteur Institute of Epidemiology and Microbiology, St. Petersburg, Russian Federation
Email: coherneph@mail.ru
PhD (Chemistry), Head of the Department of New Technology Russian Federation
A. Yu. Volkov
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian FederationMedtechnopark LTD, Moscow, Russian Federation
Email: coherneph@mail.ru
PhD (Physics and Mathematics), Senior Researcher, Laboratory of Nanotechnology in Medicine, Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine; Director of Medtechnopark LTD, Moscow, Russian Federation Russian Federation
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