Gene polymorphism and levels of pro-inflammatory cytokines in plasma and synovial fluid of patients with posttraumatic knee osteoarthritis

Abstract

Posttraumatic knee osteoarthritis patients (n = 117) and healthy individuals (n = 94) were investigated to determine the genotype and allelic frequency distribution of polymorphic loci Т-31С, С + 3953Т IL-1β and G-308A TNF-α. The plasma and synovial fluid levels of proinflammatory cytokines IL-1β and TNF-α were also measured in 31 patients. The study demonstrated that the plasma and synovial fluid levels of IL-1β but not TNF-α were increased in patients with posttraumatic knee osteoarthritis. Also, the linkage between TNF-α levels and polymorphic locus G-308A TNF-α was shown: the presence of allele–308А was associated with decreased TNF-α levels. It was found that polymorphic loci Т-31С, С+3953Т IL-1β, and G-308A TNF-α are not markers of predisposition to posttraumatic knee osteoarthritis in Russian population of Rostov Oblast.