Email this article The role of plasminogen and urokinase activator of plasminogen in the treatment of endogenous uveitis in children
- Authors: Katargina L.А.1, Chesnokova N.B.1, Denisova E.V.1, Khrabrova M.A.1, Beznos O.V.1
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Affiliations:
- Helmholtz National Medical Research Center of Eye Diseases
- Issue: Vol 18, No 2 (2023)
- Pages: 57-66
- Section: Original study article
- URL: https://journals.rcsi.science/1993-1859/article/view/148252
- DOI: https://doi.org/10.17816/rpoj321468
- ID: 148252
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Abstract
AIM: To determine the content of plasminogen and urokinase activator of plasminogen in tears and blood serum and to identify correlations between the studied parameters and the clinical picture of uveitis.
MATERIAL AND METHODS: One hundred thirty-three eyes with uveitis were examined in 74 patients aged 3 to 17 yr (average 10.45±3.35 yr). The content of the urokinase activator of plasminogen (UPA) was studied in 188 tear samples and 22 blood serum samples. The dynamics of UPA in tears were studied in 28 patients (51 eyes). The plasminogen content of 86 tear samples and 34 blood serum samples was studied. The dynamics of plasminogen in tears were studied in five patients (nine eyes). The concentrations of UPA and plasminogen were measured using the ELISA method and the kits “ELISA kit for plasminogen activator, urokinase (UPA)/ELISA Kit for Plasminogen”, Cloud-Clone Corp., USA).
RESULTS: An increase in the content of UPA in the tears of children with uveitis was associated with higher inflammatory activity (p=0.04). An increase in the content of UPA in tears was associated with an increase in the degree of proliferative changes (p=0.04). An increase in the content of UPA and plasminogen in tears was found 1–2 months after surgery. There was an increase in the content of UPA (p=0.0001) and plasminogen in tears (p=0.009) and blood serum (p=0.09) with age.
CONCLUSION: The content of UPA in tears increased significantly when severe uveitis was compared with inactive uveitis. An increase in the content of UPA in tears was associated with an increase in the degree of proliferative changes, which reflects the severity of the uveitis course. The content of UPA and plasminogen in tears and blood serum increased with age. An increase in UPA and plasminogen was observed within 1–2 months after surgery, with both returning to preoperative values by the third month of the postoperative period, which reflects the normal course of the wound healing process.
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##article.viewOnOriginalSite##About the authors
Ludmila А. Katargina
Helmholtz National Medical Research Center of Eye Diseases
Email: katargina@igb.ru
ORCID iD: 0000-0002-4857-0374
Scopus Author ID: 137428
MD, Dr. Sci. (Med.), Professor
Russian Federation, 14/19, Sadovaya Chernogryazskaya Str., 105062 MoscowNatalya B. Chesnokova
Helmholtz National Medical Research Center of Eye Diseases
Email: nchesnokova2012@yandex.ru
ORCID iD: 0000-0002-7856-8005
SPIN-code: 8705-7248
MD, Dr. Sci. (Biol.), Professor
Russian Federation, 14/19, Sadovaya Chernogryazskaya Str., 105062 MoscowEkaterina V. Denisova
Helmholtz National Medical Research Center of Eye Diseases
Email: deale_2006@inbox.ru
ORCID iD: 0000-0003-3735-6249
SPIN-code: 4111-4330
MD, PhD Researcher, Department of children’s eye pathology
Russian Federation, 14/19, Sadovaya Chernogryazskaya Str., 105062 MoscowMariia A. Khrabrova
Helmholtz National Medical Research Center of Eye Diseases
Email: khrabrovamaria@mail.ru
ORCID iD: 0000-0001-9422-4264
MD, graduate student
Russian Federation, 14/19, Sadovaya Chernogryazskaya Str., 105062 MoscowOlga V. Beznos
Helmholtz National Medical Research Center of Eye Diseases
Author for correspondence.
Email: olval2011@mail.ru
ORCID iD: 0000-0001-7557-4955
SPIN-code: 7894-5162
MD, Doctor
Russian Federation, 14/19, Sadovaya Chernogryazskaya Str., 105062 MoscowReferences
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