Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae


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Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

Sobre autores

A. Zhuk

St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University

Autor responsável pela correspondência
Email: ania.zhuk@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034

E. Stepchenkova

St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University

Email: ania.zhuk@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034

Y. Pavlov

University of Nebraska Medical Center

Email: ania.zhuk@gmail.com
Estados Unidos da América, Omaha, 68198

S. Inge-Vechtomov

St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University

Email: ania.zhuk@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034

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