Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae
- Autores: Zhuk A.S.1,2, Stepchenkova E.I.1,2, Pavlov Y.I.3, Inge-Vechtomov S.G.1,2
-
Afiliações:
- St. Petersburg Branch of N.I. Vavilov Institute of General Genetics
- St. Petersburg State University
- University of Nebraska Medical Center
- Edição: Volume 11, Nº 2 (2017)
- Páginas: 111-122
- Seção: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212302
- DOI: https://doi.org/10.1134/S1990519X17020110
- ID: 212302
Citar
Resumo
Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).
Sobre autores
A. Zhuk
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Autor responsável pela correspondência
Email: ania.zhuk@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034
E. Stepchenkova
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Email: ania.zhuk@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034
Y. Pavlov
University of Nebraska Medical Center
Email: ania.zhuk@gmail.com
Estados Unidos da América, Omaha, 68198
S. Inge-Vechtomov
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Email: ania.zhuk@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034
Arquivos suplementares
