Email this article Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae
- Authors: Zhuk A.S.1,2, Stepchenkova E.I.1,2, Pavlov Y.I.3, Inge-Vechtomov S.G.1,2
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Affiliations:
- St. Petersburg Branch of N.I. Vavilov Institute of General Genetics
- St. Petersburg State University
- University of Nebraska Medical Center
- Issue: Vol 11, No 2 (2017)
- Pages: 111-122
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212302
- DOI: https://doi.org/10.1134/S1990519X17020110
- ID: 212302
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Abstract
Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).
About the authors
A. S. Zhuk
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Author for correspondence.
Email: ania.zhuk@gmail.com
Russian Federation, St. Petersburg, 199034; St. Petersburg, 199034
E. I. Stepchenkova
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Email: ania.zhuk@gmail.com
Russian Federation, St. Petersburg, 199034; St. Petersburg, 199034
Y. I. Pavlov
University of Nebraska Medical Center
Email: ania.zhuk@gmail.com
United States, Omaha, 68198
S. G. Inge-Vechtomov
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Email: ania.zhuk@gmail.com
Russian Federation, St. Petersburg, 199034; St. Petersburg, 199034
