Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae
- 作者: Zhuk A.1,2, Stepchenkova E.1,2, Pavlov Y.3, Inge-Vechtomov S.1,2
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隶属关系:
- St. Petersburg Branch of N.I. Vavilov Institute of General Genetics
- St. Petersburg State University
- University of Nebraska Medical Center
- 期: 卷 11, 编号 2 (2017)
- 页面: 111-122
- 栏目: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212302
- DOI: https://doi.org/10.1134/S1990519X17020110
- ID: 212302
如何引用文章
详细
Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).
作者简介
A. Zhuk
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
编辑信件的主要联系方式.
Email: ania.zhuk@gmail.com
俄罗斯联邦, St. Petersburg, 199034; St. Petersburg, 199034
E. Stepchenkova
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Email: ania.zhuk@gmail.com
俄罗斯联邦, St. Petersburg, 199034; St. Petersburg, 199034
Y. Pavlov
University of Nebraska Medical Center
Email: ania.zhuk@gmail.com
美国, Omaha, 68198
S. Inge-Vechtomov
St. Petersburg Branch of N.I. Vavilov Institute of General Genetics; St. Petersburg State University
Email: ania.zhuk@gmail.com
俄罗斯联邦, St. Petersburg, 199034; St. Petersburg, 199034