Growth and Development of Isolated Tips of Neurospora crassa Hyphae in a Sorbitol-Containing Medium
- Authors: Potapova T.V.1, Boitsova L.Y.1, Golyshev S.A.1, Dunina-Barkovskaya A.Y.1, Belozerskaya T.A.2,3
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Affiliations:
- Belozersky Research Institute of Physicochemical Biology
- Faculty of Biology
- Bach Institute of Biochemistry
- Issue: Vol 12, No 3 (2018)
- Pages: 256-263
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212684
- DOI: https://doi.org/10.1134/S1990519X18030100
- ID: 212684
Cite item
Abstract
It is known that the replacement of easily utilizable glucose with sorbitol in a medium activates a large number of repressed genes and metabolic changes in Neurospora crassa, which manifest themselves at the cellular level in a considerable vacuolization of the cytoplasm. The study of the morphological parameters and growth characteristics of isolated apical fragments of N. crassa hyphae showed that, in sorbitol-containing medium, the isolation from the parent mycelium causes the same disturbances in the coordination of elongation, branching, and septation as those described by us earlier for the glucose-containing medium. The metabolic changes caused by the carbon source replacement do not affect the hyphal-segment size, the distance from the growth point to the first septum, and the morphology and distribution of nuclei.
About the authors
T. V. Potapova
Belozersky Research Institute of Physicochemical Biology
Author for correspondence.
Email: potapova@genebee.msu.su
Russian Federation, Moscow, 119234
L. Yu. Boitsova
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Russian Federation, Moscow, 119234
S. A. Golyshev
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Russian Federation, Moscow, 119234
A. Ya. Dunina-Barkovskaya
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Russian Federation, Moscow, 119234
T. A. Belozerskaya
Faculty of Biology; Bach Institute of Biochemistry
Email: potapova@genebee.msu.su
Russian Federation, Moscow, 119192; Moscow, 119071