Growth and Development of Isolated Tips of Neurospora crassa Hyphae in a Sorbitol-Containing Medium
- Авторлар: Potapova T.1, Boitsova L.1, Golyshev S.1, Dunina-Barkovskaya A.1, Belozerskaya T.2,3
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Мекемелер:
- Belozersky Research Institute of Physicochemical Biology
- Faculty of Biology
- Bach Institute of Biochemistry
- Шығарылым: Том 12, № 3 (2018)
- Беттер: 256-263
- Бөлім: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212684
- DOI: https://doi.org/10.1134/S1990519X18030100
- ID: 212684
Дәйексөз келтіру
Аннотация
It is known that the replacement of easily utilizable glucose with sorbitol in a medium activates a large number of repressed genes and metabolic changes in Neurospora crassa, which manifest themselves at the cellular level in a considerable vacuolization of the cytoplasm. The study of the morphological parameters and growth characteristics of isolated apical fragments of N. crassa hyphae showed that, in sorbitol-containing medium, the isolation from the parent mycelium causes the same disturbances in the coordination of elongation, branching, and septation as those described by us earlier for the glucose-containing medium. The metabolic changes caused by the carbon source replacement do not affect the hyphal-segment size, the distance from the growth point to the first septum, and the morphology and distribution of nuclei.
Негізгі сөздер
Авторлар туралы
T. Potapova
Belozersky Research Institute of Physicochemical Biology
Хат алмасуға жауапты Автор.
Email: potapova@genebee.msu.su
Ресей, Moscow, 119234
L. Boitsova
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Ресей, Moscow, 119234
S. Golyshev
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Ресей, Moscow, 119234
A. Dunina-Barkovskaya
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Ресей, Moscow, 119234
T. Belozerskaya
Faculty of Biology; Bach Institute of Biochemistry
Email: potapova@genebee.msu.su
Ресей, Moscow, 119192; Moscow, 119071