Growth and Development of Isolated Tips of Neurospora crassa Hyphae in a Sorbitol-Containing Medium
- Autores: Potapova T.1, Boitsova L.1, Golyshev S.1, Dunina-Barkovskaya A.1, Belozerskaya T.2,3
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Afiliações:
- Belozersky Research Institute of Physicochemical Biology
- Faculty of Biology
- Bach Institute of Biochemistry
- Edição: Volume 12, Nº 3 (2018)
- Páginas: 256-263
- Seção: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212684
- DOI: https://doi.org/10.1134/S1990519X18030100
- ID: 212684
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Resumo
It is known that the replacement of easily utilizable glucose with sorbitol in a medium activates a large number of repressed genes and metabolic changes in Neurospora crassa, which manifest themselves at the cellular level in a considerable vacuolization of the cytoplasm. The study of the morphological parameters and growth characteristics of isolated apical fragments of N. crassa hyphae showed that, in sorbitol-containing medium, the isolation from the parent mycelium causes the same disturbances in the coordination of elongation, branching, and septation as those described by us earlier for the glucose-containing medium. The metabolic changes caused by the carbon source replacement do not affect the hyphal-segment size, the distance from the growth point to the first septum, and the morphology and distribution of nuclei.
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Sobre autores
T. Potapova
Belozersky Research Institute of Physicochemical Biology
Autor responsável pela correspondência
Email: potapova@genebee.msu.su
Rússia, Moscow, 119234
L. Boitsova
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Rússia, Moscow, 119234
S. Golyshev
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Rússia, Moscow, 119234
A. Dunina-Barkovskaya
Belozersky Research Institute of Physicochemical Biology
Email: potapova@genebee.msu.su
Rússia, Moscow, 119234
T. Belozerskaya
Faculty of Biology; Bach Institute of Biochemistry
Email: potapova@genebee.msu.su
Rússia, Moscow, 119192; Moscow, 119071