The effect of quercetin on hepatic differentiation of human adipose-derived mesenchymal stem cells
- Authors: Pinchuk S.V.1, Vasilevich I.B.1, Kvacheva Z.B.1, Volotovski I.D.1
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Affiliations:
- Institute of Biophysics and Cell Engineering
- Issue: Vol 10, No 5 (2016)
- Pages: 357-364
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212159
- DOI: https://doi.org/10.1134/S1990519X16050102
- ID: 212159
Cite item
Abstract
Human adipose-derived mesenchymal stem cells (MSCs) can be stimulated to differentiate into hepatic cells. MSC differentiation was induced by fibroblast growth factor-4, hepatocyte growth factor, oncostatin M, and dexamethasone. The influence of quercetin on MSC hepatic differentiation in culture was assayed, and 1 or 10 μmole/L quercetin added into the induction medium enhanced the manifestation of MSC hepatic differentiation. Urea secretion, cytokeratin 19 expression, and α-fetoprotein synthesis were increased. Quercetin modulated CYP1A–cytochrome P450 activity in the differentiated cells. MSCs differentiated in the presence of quercetin exhibited higher viability and resistance to oxidative stress.
About the authors
S. V. Pinchuk
Institute of Biophysics and Cell Engineering
Author for correspondence.
Email: pinchuksv@mail.ru
Belarus, Minsk, 220072
I. B. Vasilevich
Institute of Biophysics and Cell Engineering
Email: pinchuksv@mail.ru
Belarus, Minsk, 220072
Z. B. Kvacheva
Institute of Biophysics and Cell Engineering
Email: pinchuksv@mail.ru
Belarus, Minsk, 220072
I. D. Volotovski
Institute of Biophysics and Cell Engineering
Email: pinchuksv@mail.ru
Belarus, Minsk, 220072