Study of Antioxidant Activity and Composition of Cyanobacteria Metabolites by TLC, HPTLC, and HPLC for the Search of Environmentally Safe Cleaning Agents

Abstract

The composition of metabolites of cyanobacteria cultures of the Astrakhan region has been studied by thin-layer chromatography, high-performance thin layer chromatography, and high-performance liquid chromatography. Thin layer chromatography analysis of the cyanobacteria metabolites has shown the best separation and quality of chromatographic zones in seven systems: chloroform, ethanol: acetic acid (1: 1), butanol, ethanol: water (1: 9), butanol: acetic acid: water (4: 1: 5), ethanol: water (9: 1), and butanol: acetic acid: water (4: 1: 5). The flavonoid quercetin has been detected in an aqueous–alcoholic extract by high -performance thin-layer chromatography. The high-performance liquid chromatography has allowed identification of aspartic, isomonic, lactic, and fumaric acids in the cyanobacteria metabolites. Antioxidant activity of aqueous and aqueous-alcoholic extracts of the Phormidium ramosum B.-Peters IPPAS B-2022 culture has been detected.