


Vol 44, No 5 (2018)
- Year: 2018
- Articles: 20
- URL: https://journals.rcsi.science/1068-3674/issue/view/14276
Crop Production
Blending Value of Winter Rye with Various Viscosity of an Aqueous Extract of Grain Meal
Abstract
The wide use of rye flour in the form of rye-wheaten and wheaten-rye mixes has caused the problem of the blending value of cultivated varieties, which is as important for rye as for wheat. The purpose of the research was to comparatively study the blending value of winter rye samples with various viscosity of aqueous extract of grain meal. Six rye samples with low viscosity (2.1–7.3 cP) as recipients and three samples with high viscosity (10.0–15.0 cP) were used as donors. Recipient and donor grains were mixed in the ratio 4: 1, 3: 2, 2: 3, and 1: 4. The following traits were studied: viscosity of aqueous extract (VAE), height of amylogram, falling number, shape stability of a toppling bread (H/D ratio), and volume of pan bread. Blending value of components was estimated based on improvement effect (E). It was shown that viscosity and other quality traits correlatively associated with it were subjected to the mixing rule: their expression naturally increased with increased proportion of the highly viscous donor in the mixture. The exception was the volume of pan bread, the expression of which decreased in this case. During mixing, the actual values of the traits in the mixtures almost completely coincided with the calculated values. Significant deviations from the weighted average values were revealed only for amylogram height and the volume of the pan bread, indicating possible interaction effects of the mixed components. A negative correlation was found between the viscosity level of the recipient and the effect of its improvement (r =–0.93). The most valuable improver in mixtures with low viscous rye was the Moc-12-BB donor, characterized by high expression of the VAE (15.0 cP). It was concluded that such rye could be classified as strong since a lower amount of this rye should be added to the mixture for improvement of the weak (low-viscosity) rye. The addition of highly viscous rye to low viscous rye in an amount of 20–40% was the most preferable.



High-Carotinoid Triticale: Breeding and Use
Abstract
It is shown that the concept of carotene includes several substances (carotene, xanthophyll and its ethers), which play an important role in the metabolism of photosynthetic plants and then in the storage of seeds. They are of no less importance for the technological properties of grain and the products of its processing, for human nutrition. The nature of the inheritance of this property turned out to be peculiar: for example, heterosis was observed in 7% of the combinations, depression in 41%, and intermediate inheritance in the remaining. Highly carotenoid (HC) recombinants of transgressive triticale were isolated from populations with heterosis in F1 and prolonged formation. The possibility of increasing the intensity of HC by backcrosses with donors of HC was determined. The dominant role of HC donors was revealed. Also, the creation of such triticale is possible with the help of chemical mutagenesis. Technologies for the preparation of flour, different cereals, and flakes have been developed. At the same time, the concentration of carotenoids of the HC triticale was revealed mainly in the endosperm of the grain, which makes it possible to increase their percentage content in grain products (cereals, flour, etc.), as well as products from them by 13% on average. Products from the predominant number of HC triticale (winter and spring) are not inferior in quality to the spaghetti prepared from spring durum wheat.



Fatty Acid Composition of Lipids in Naked Oat Grain of Domestic Varieties
Abstract
Naked oat lines 1h07, 766h05, 857h05, 41h04, 37h12о, 74h12, and 8h12 have been studied to characterize the fatty acid composition of acyl lipids, which represent one of the basic groups of macronutrients providing energy and plastic material for metabolic processes. Crude lipid content in the grain of these lines is 6.9 ± 098% on average and varies from 5.91 to 7.87%. Fatty acids dominating in the lipid composition of the grain include palmitic (15.3–17.8%), oleic (33.5–36.7%), and linoleic (36.2–38.7%) acids. According to the content of oleic and linoleic acide and their ratio (1: 1), lipids of naked oat belong to the oleic-linoleic group of vegetable oils.



Change in Quality Parameters of Hulled Oats Grain at Fractionation
Abstract
The changes in the main indices for grain quality of hulled oats at fractionation for the development of the method for their regulation were studied. It was shown that the grains of various fractions of hulled oats is not equal by biochemical composition and technological qualities. Fractionation of the grain samples collected from homogeneous large batches of oats and carrying out of corresponding analyses allowed the determination of a broad range of quality indices of individual fractions and their dependence on the thickness of the caryopsis. Then, by the correct selection and installation of top and (or) bottom sieves on seed cleaning machines of secondary cleaning, one can change (increase or decrease) the basic quality indices of a batch of grain from hulled oats up to values regulated by the State Standard or standards demanded by the consumer.



Effect of Polyketides from the Marine Fungus Penicillium thomii Maire KMM 4675 on Productivity and Growth of Spring Barley Sprouts
Abstract
Eleven polyketides that have been tested in laboratory and field conditions as growth stimulants of the spring barley (Hordeum vulgare L.) were educed from marine fungus Penicillium thomii Maire KMM 4675, which was obtained from mycobiota of the brown seaweed Sargassum pallidum (Novik Bay, Russky Island, Sea of Japan). Depending on the chemical structure, polyketides have different effects on the growth of barley sprouts. It is shown that the compounds pallidopenilline A, 1-acetoxy-pallidopenilline A, 1-deacetyl- pallidopenilline B, pallidopenilline E, pallidopenilline F, and pallidopenilline H have a stimulating effect on the growth of sprout roots (from 2 to 24%) and the compounds pallidopenilline A and 1-acetoxy-pallidopenilline A stimulate the productivity of the spring barley at concentrations of 1.0 μg/mL (17.1%) and 10.0 μg/mL (19.5%), respectively. Pallidopenilline A and its acetylized derivative 1-acetoxy-pallidopenilline A showed the maximum stimulatory effect (24 and 10%, respectively) on the growth of barley sprouts at a concentration of 0.01 μg/mL. Pallidopenilline A is recommended for wider use in the field conditions as a growth stimulant.



The Role of Microbiological Fertilizers and Green Manures in Increasing the Productivity and Quality of Potatoes
Abstract
This paper presents the research results of three short-term experiments carried out in 2010–2017 on the impact of green manures and mineral and bacterial fertilizers on potato productivity. The purpose of the study was to increase the productivity and quality of potato tubers while reducing the anthropogenic load on the agrocenosis through the integrated use of binary green manures and lower doses of mineral fertilizers in combination with microbiological preparations. In a stationary field experiment (2010–2012) on leached chernozem, the maximum potato productivity (32.2–35.5 t/ha) was obtained using mineral fertilizers N45–90P60–120K60–120 in the crop rotation link oil radish + vetch–potato, while the efficiency of the treatment of tubers with the bacterial preparations Azotovit + Fosfatovit was 9.2–15.0%. In experiments on sod-podzolic soil, the yield increase from the microbiological preparation Agrinos for the potato cultivar Udacha in 2015 amounted to 12.4% along with higher content of starch and vitamin C in production. In the relatively moist 2016 and 2017, the yield increase in the midearly maturing cultivar Gala reached 8.5 t/ha or 24.5% to the mineral background level (N90P90K90). In the variant with a 30% reduced NPK dose and the use of Agrinos (N60P60K60 + Agrinos A + B (5 + 2.5 L/ha), the yield was 5.8 t/ha or 16.7% higher than with the full dose of NPK, and high starchiness and vitamin C content, excellent culinary qualities, and low levels of nitrates were also observed.



Traditional and Innovative Approaches in the Breeding of Garden Chrysanthemum in the South of the Russian Far East
Abstract
This paper defines priority plant-breeding-significant features and properties of new varieties that differ from native and foreign analogues in complex adaptability to biotic and abiotic stresses of the monsoon climate of the Russian Far East and high decorativeness. Traditional and innovative approaches in the breeding of the garden chrysanthemum are presented. Intervarietal, interspecific, and closely related crosses, as well as radiation mutagenesis, were used. Interspecific hybridization based on adaptive Manchurian and Korean high-mountain species of the Chrysanthemum genus made it possible to improve the genetic basis of existing varieties and solve the problem of individual and complex economic features. The genotypes that combine the main target features of immunity to white rust, winter hardiness, and optimal flowering period are created for the first time in the native breeding of the garden chrysanthemum. Hybrids and varieties of a new generation are obtained, which make it possible to expand the domestic assortment.



Effects of Root-Zone Temperature on Growth, Chlorophyll Fluorescence Characteristics and Chlorophyll Content of Greenhouse Pepper Plants Grown under Cold Stress in Southern China
Abstract
In Southern China, plants are usually exposed to cold stress during winter in an unheated greenhouse, but due to the high energy consumption and costs, most of the greenhouses remain unheated. In an attempt to find a simple and affordable solution to this problem, this study was undertaken. In this research, Capsicum frutescens L. plants were studied to investigate the effect of different root zone temperatures on its growth and chlorophyll fluorescence characteristics under cold stress. The plants were cultivated under cold stress conditions in a root zone temperature (RZT) control system where the roots were subjected to four different root-zone temperature treatments of 20°C-T20, 25°C-T25, 45°C-T45 and a control CK group. Growth characteristics studied included plant height, stem diameter, plant width, root length, biomass accumulation. Whilst fluorescence characteristics investigated were chlorophyll fluorescence ratio Fv/Fm, photochemical quenching (qL), efficiency of Photosystem II (Y[II]) and electron transport rate (ETR). Chlorophyll content in the leaves of the plants was also investigated. The findings demonstrated that plants in the CK group suffered a detrimental effect on the growth characteristics registering the lowest values in the measured variables. Conversely, the highest values were observed in T25 RZT treatment. In fluorescence characteristics, values of Fv/Fm were maintained at between 0.8 and 0.83 but also suffered a photo-inhibitory depression in CK and T45 RZT treatments to Fv/Fm values of <0.79. This depicted that root zone heating protected the PS II of these plants from photoinactivation induced by cold stress. Similar trends were seen in the qL, Y[II], ETR values with the T20 and T25 treatments registering the highest values. Chlorophyll content was significantly higher in the leaves of the plants in the T20 and T25 group. The lowest chlorophyll content was recorded in the CK group. Plants in all the treatments accumulated more biomass in the shoot than in the roots as depicted by a significantly lower shoot to root ratio values with the exception of those in the CK group. The findings of this study suggest that pepper plants can successfully be grown in an unheated greenhouse in the Yangtze River Delta area of Southern China during winter by heating the root zone of the plants to a RZT value of 25°C, thereby providing a simple, affordable and cost-effective technique.



Implications of Gene Actions for Different Qualitative and Quantitative Traits in Linseed (Linum usitatissimum L.)
Abstract
The ninety crosses were evaluated for 27 qualitative and quantitative traits to study the different gene actions, genetic variances, heritability and genetic advances involved in parents and in their off springs. The analysis of variance showed highly significant differences present among all the treatments for all quantitative and qualitative traits. The estimates of genetic component of variances together with related genetic parameters indicated that the magnitude of SCA variances was more pronounced than their corresponding GCA variances for majority of the traits for all testers and lines in both generations which suggested the role of non-additive gene action in the inheritance of these traits. The information obtained from the present study exhibited most of the traits had high value of σ2s than σ2g and over dominance gene action so, it is necessary to maintain the heterozygosity in the population for the improvement purposes. The non-additive genetic variability is non-fixable in nature thus the breeding methods such as biparental mating followed by recurrent selection may accelerate the genetic improvement for the traits sought to be improved.



Plant Protection
Phytosanitary Means of Pest Control in Grain Crops
Abstract
The results of studying the changes in the range of insecticides for grain crop protection are presented for the period of more than 20 years. The aspects of extension are assessed for the range of insecticides used for these purposes, the peculiarities of certain chemical classes are shown along with the terms of pesticide application against various types of pests, and the dynamics of the ecological and toxicological properties of the pesticides are reviewed from 1995 up to the present day. The range of plant protection products is analyzed for the period of 1995–2017. It is shown that these changes are a continuous process that is expressed both as an increase in the total number of pesticides and as a qualitative improvement by introducing the new ones or combining the active substances of different content and formulations as well as by improving technologies and methods of pesticide application.



Identification of Habrobracon hebetor Populations Using RAPD Markers
Abstract
It was found that food preferences and conditions of breeding of Habrobracon hebetor laboratory populations vary considerably. In this regard, it is necessary to identify populations within the studied species using DNA markers: an effective and reliable means for assessing the genetic differences between samplings of this insect species. A molecular genetic analysis of two different geographic populations of the Habrobracon hebetor entomophage (from Krasnodar, Russia, and Chimkent, Kazakhstan) was carried out using RAPD markers; 21 RAPD primers were tested for specificity to H. hebetor DNA. Five RAPD primers (OPA05, OPA10, OPB01, OPB04, and UBC519) were identified that have high specificity and the ability to differentiate H. hebetor populations. DNA markers that are specific for the Krasnodar and Chimkent entomophage populations and that can clearly identify them were revealed: for the Krasnodar population, RAPD markers with a molecular weight of 550 bp (UBC 519); 500 and 700 bp (OPA05); 1100, 1200, and 1300 bp (OPA10); 220 and 800 bp (OPB04); and 880 bp (OPB01); for the Chimkent population, 400, 600 and 1200 bp (UBC519); 600 and 950 bp (OPA10); and 800 bp (OPB01). It is concluded that these RAPD primers can be used for identification and differentiation of other H. hebetor populations.



Agrochemistry. Soil Science
Dynamics of the Typical Chernozems’ Properties in the Streletskaya Steppe National Park under the Influence of Long-Term Agrogenic Impact
Abstract
Typical chernozems of protected areas are characterized by a high humus content, a stable absorbing complex, and favorable agrochemical properties and demonstrate high number and activity of different groups of microorganisms, which is indicative of deep mineralization of plant residues and the storage of biogenic elements in microbial biomass. In the longstanding fallows, the structure of the microbial community is disturbed: the proportion of the oligotrophic groups, which are able to transform humic substances, increases. The rate of cellulose decomposition slows down, phytotoxicity increases, and dehumification develops. In the rotation, negative dynamic of these indicators is smoothed.



Trends of Soil Processes in Natural Landscapes of Mountain and Piedmont Provinces of Dagestan
Abstract
Data of long-term researches for revealing the trend of modern processes of soil formation and degradation in the mountain and piedmont provinces of Dagestan are given. The aim of the researches was to study the modern soil-erosion and ecologic-ameliorative status of the soil cover and changes in the naturalresource potential of slope lands under the effect of natural and anthropogenic factors over a 50-year period. A strong effect of exogenic and endogenic factors on slope ecosystems results in development of erosion and degradation processes. The soil cover is formed under conditions of intensive geomorphologic, geochemical, and biological processes. The increasing anthropogenic load and climate aridization exert a strong effect on their dynamics. The comparison of data of earlier soil-erosion survey with modern ones shows great changes in the spatial pattern of eroded soils. The areas of noneroded lands became two times smaller over the period of studies. There is a tendency for a decrease in the areas of brown forest soils of a hydromorphic type of soil formation and for enlargement in the area of semihydromorphic and automorphic brown and chestnut soils. A great diversity of soil–plant cover in the areas with vertical zonality is related to differences in slope aspects, topography, and steepness. Soil evolution is characterized by a trend from hydromorphic and semihydromorphic to automorphic stages of soil formation.



Animal Husbandry
Association of Leptin Gene (LEP) Polymorphism with Growth Rates and Milk Production in Holstein First-Calf Heifers
Abstract
The objective of the work is to study the polymorphic leptin gene variants and their influence on growth, development, and milk production of Holstein first-calf heifers, whose 172 blood samples were analyzed. Genotyping the leptin gene locus was performed with the PCR-RFLP method. The frequencies of occurrence of the C and T alleles comprised 0.62 and 0.38, respectively; the values for genotypes CC, TC, and TT comprised 35.5% (61 animals), 53.3% (90 animals), and 12.2% (21 animals), respectively. It was ascertained that the analyzed animal population is in the genetic balanced equilibrium state according to the Hardy–Weinberg law. Under similar conditions of feeding and nutritional supplement, the animals having genotype CC were significantly superior to their peers with the other genotypes in all the parameters characterizing the live weight at different ages. However, the milk yield, the fat mass fraction, the milk fat yield, and the milk protein yield in the specimens having the TT genotype were higher by 8.9% (P ≤ 0.01; 673.4 kg), 0.20, 12.9% (P ≤ 0.05; 38.4 kg), and 9.0% (P ≤ 0.05; 22.7 KG), respectively, than that in the genotype TC carriers. The coefficient of milkability was 142.1 kg higher in all the first-calf heifers when compared to the lower value for the entire herd, therefore, indicating the specific dairy type of the analyzed animal population. The obtained data can prove the associations between the different genotypes of the leptin gene and the growth rates, the physical development, and the milk production in Holstein cattle.



Molecular and Biochemical Aspects of the Ontogenesis of Eukaryotes Under Global Warming
Abstract
Global warming is an irreversible process resulting in the deterioration of living conditions for various organisms, including the most important agricultural species. So-called σ32 factor of Escherichia coli is embedded into the RNA thermosensor in the λ cIII gene and plays an important role in the regulation of bacterial response to heightened temperatures. Expression of heat/cold shock genes and some virulence genes in response to temperature changes is coordinated by the genome. There are some known RNA thermosensors with different structures that provide a functional control of the diversity of cellular processes. The most common RNA thermosensor is the ROSE element suppressing expression of heat shock genes. A common feature of all ROSE elements is the presence of the G residue opposite to the SD sequence since this nucleotide is functionally important and its elimination makes the RNA thermosensor insensitive to high temperatures. In this paper, we describe molecular sequences (RNA thermosensors) whose chemical compounds influence on the homeostatic temperature regulation, namely, on the corresponding enzymes. Though the data on RNA thermosensors were obtained for microorganisms, it may be possible in the long run to change the animal genome at the molecular level by the insertion of these sequences or cultivation of symbiotic microorganisms, which may be used for production of biologically active compounds. In addition, such insertions would probably be able to reduce the negative effect of high environmental temperatures on living organisms.



Veterinary
Biological Properties and Molecular-Genetic Characteristics of African Swine Fever Virus Isolated in Various Regions of Russia in 2016–2017
Abstract
African swine fever virus (ASFV) isolated in 2016–2017 from domestic pigs and wild boars in eight regions of the Russian Federation has comparable biological characteristics: it is virulent, causes the death of animals with signs of acute and overactive forms without the manifestation of a complete symptom complex of clinical and pathologic symptoms, possesses hemadsorption and accumulates in the initial (swine leukocytes, SBM) and transplantable (A4C2/9k) cell cultures in titres 5.5–7.5 and 3.5–5.25 log HAU/50cm3, respectively. The studied isolates belong to the eighth serotype and the second genotype. According to these indicators, the ASF virus is comparable with the virus isolates circulating in Russia since 2007.



Effects of Hirudotherapy on Parameters of Plasma Hemostasis in Cows with Clinical Mastitis
Abstract
The objective of the work was to study the effects of hirudotherapy on the hemostasis plasma link in cows with clinical mastitis. The blood test for 20 lactating Ayrshire cows was performed. The most active aggregation after hirudotherapy was recorded for the collagen-induced aggregation. Platelet disaggregation capabilities decreased with the impacts of adenosine diphosphate (ADP) and ristomycin. A significant decrease in the activated partial thromboplastin time (APTT) (P ≤ 0.01), the prothrombin time (PTT) (P ≤ 0.01), and the thrombin time (TT) was revealed. The use of medical leeches in the treatment of the clinical bovine mastitis did not prove any significant effect on the adhesive-aggregation activity of platelets. However, it had a corrective effect on the plasma-coagulation link of hemostasis.



Avian Leukosis in Brahma Chicken Raised in Al-Qurnah Town, Southern Iraq
Abstract
The current study was conducted to diagnose avian leukosis in naturally infected Brahma backyard chickens in southern parts of Iraq, on the basis of clincopathological findings and serological detection by using antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) in suspected tumor cases in field conditions. In this study the avian leukosis was mostly observed in birds from 16 to 22 weeks of age, as well as the infected flocks showed a low mortality rate ranging from 5–6%. Typical variable sized grey to yellow obvious tumor-like nodular lesion was demonstrated on the surface of enlarged visceral organs such as liver, spleen, kidney and duodenum, as in white meat-type chickens. The histopathological features revealed massive infiltration of monomorphic lymphocytes in which the lymphoblasts were predominant in the liver, kidney, spleen and duodenum. In this study, a total of 40 sera were tested for ALV P27 antigen by ELISA technique. Thirty-five out of forty sera (87.5%) obtained from Brahma chickens tested positive to ALV P27 antigen and a higher percentage (88.58%) of the chicken sera were strongly positive and had (EUs > 75%). Based on these findings, avian leukosis was concluded to be associated with this pathological condition in Iraqi backyard flocks. This is the first report of the presence of the avian leukosis in visceral samples of Brahma breed. It seems that commercial poultry population in Iraq is not far from the threat of the avian leukosis, and surveillance for avian leukosis is needed.



Storage and Processing of Agricultural Products
Perfection of the Technology for Production of Canned Apple Compotes Using Secondary Resources and Intensive Sterilization Regimes
Abstract
This paper presents the results of research on improving the technology for production of canned apple compotes using secondary products after cutting and cleaning fruit for syrup boiling and intensive heat sterilization based on the use of new techniques—preliminary heating of fruit in cans in the ultrahigh frequency electromagnetic field with sterilization under intensive regimes with the use of high-temperature heat carriers and rotation of the cans during sterilization. It is established that the use of the resulting infusion of secondary products for syrup boiling provides saving of more than 8 kg of sugar per tube of product. In this case, the content of vitamin C in the finished product is more than two times higher than in the case of using the traditional method of blanching in hot water. Studies on the heating and actual lethality of microorganisms have shown that the developed mode provides the industrial sterility of canned food, saves heat energy, and shortens the duration of the heat sterilization mode by 20 min.



Mechanization
High-Frequency Electric Shearing Unit for Sheep
Abstract
A comparative analysis of sheep-shearing machines has been performed. Advantages and disadvantages of shearing machines with a single-phase commutator motor compared to a three-phase asynchronous electric motor with a short-circuited rotor are identified. An individual electric shearing unit with the improved converter and upgraded cutting machine with a vertical push mechanism is offered. This assembly equipment increases the efficiency of shearing the sheep due to the improved characteristics of the converter and the working parts of the shearing machine. The frequency converter has an energy saving function reducing the energy losses. The weight of the improved converter compared to the prototype is decreased by 2.1 kg. The vertical push mechanism gives a threefold reduction in the uneven pressing of the knife to the comb in a sharing machine. Comparative analysis of sheep-shearing machines has been performed. Advantages and disadvantages of shearing machines with a single-phase commutator motor compared to the three-phase asynchronous electric motor with a short-circuited rotor are identified. An individual electric shearing unit with the improved converter and upgraded cutting machine with a vertical push mechanism is offered. This assembly equipment increases the efficiency of shearing the sheep due to the improved characteristics.


