Antiviral Activity of a New Class of Chemically Modified Antisense Oligonucleotides against Influenza А Virus
- Авторы: Markov A.1, Kupryushkin M.1, Goncharova E.1, Amirkhanov R.1, Vasilyeva S.1, Pyshnyi D.1, Zenkova M.1, Logashenko E.1
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Учреждения:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- Выпуск: Том 45, № 6 (2019)
- Страницы: 774-782
- Раздел: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229339
- DOI: https://doi.org/10.1134/S1068162019060268
- ID: 229339
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Аннотация
The paper reports the synthesis of a series of antisense oligonucleotides (aONs) directed against different segments of the influenza A virus genome (H1N1) and screening of their antiviral activity in the influenza A virus (H1N1)/MDCK cells and influenza A virus (H1N1)/A549 cells systems. The results of screening have shown that PB1-2AUG-R aON targeted towards the AUG codon region of the segment 2 of virus genome possessed the highest antiviral activity. The synthesized morpholino analog (PMO) and the new phosphoryl guanidine oligodeoxyribonucleotide (PGO) with the sequence of oligonucleotide PB1-2AUG-R provided a comparable biological effects: the influenza virus titer in MDCK cell culture was reduced by 15 times compared to the control when PGO was used in the concentration of 10 µM and by 40 times when PMO was used in the concentration of 20 µM. For the delivery of electrically neutral analogs of oligonucleotides (PGO and PMO), the perforation method proposed for PMO was used.
Об авторах
A. Markov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
M. Kupryushkin
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
E. Goncharova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
R. Amirkhanov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
S. Vasilyeva
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
D. Pyshnyi
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
M. Zenkova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090
E. Logashenko
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Автор, ответственный за переписку.
Email: evg_log@niboch.nsc.ru
Россия, Novosibirsk, 630090