Polyamidoamine dendrimers with different surface charge as carriers in anticancer drug delivery


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Second-generation (G2) polyamidoamine (PAMAM) dendrimers are branched polymers containing 16 surface primary amine groups. Due to their structural properties, these polymers can be used as universal carriers in various drug delivery systems. Amine-terminated PAMAM dendrimers are characterized by a high positive surface charge, leading to effective but nonspecific interactions with negatively charged cell plasmatic membranes. To reduce the nonspecific internalization of PAMAM dendrimers, their primary amine groups are often modified by acetic or succinic anhydrides, polyethylene glycol derivatives and other compounds. In this work, the role of primary amine groups, which are localized on the surface of doxorubicin-conjugated (Dox) dendrimers, was studied with regard to their intracellular distribution and internalization rates using SKOV3 human ovarian adenocarcinoma cells. It was demonstrated that all Dox-labeled G2-derivatives containing different numbers of acetamide groups synthesized in this work show high rates of cellular uptake at 37°С. As expected, the conjugate carrying the maximum number of primary amine groups demonstrated the highest rates of binding and endocytosis. At the same time, the G2-Dox conjugate containing the maximum number of acetamide groups showed colocalization with LAMP2, a marker of lysosomes and late endosomes, as well as the highest level of cytotoxic activity against SKOV3 cells. We conclude that second-generation PAMAM dendrimers are characterized by varied pathways of internalization and intracellular distribution due to the number of primary amine groups on their surface and, as a consequence, a different surface charge.

Sobre autores

N. Yabbarov

Tsyb Medical Radiological Research Centre, Branch of the National Medical Research Radiological Center

Autor responsável pela correspondência
Email: nikita_yabbarov@yahoo.com
Rússia, Obninsk, 249036

E. Nikolskaya

Tsyb Medical Radiological Research Centre, Branch of the National Medical Research Radiological Center

Email: nikita_yabbarov@yahoo.com
Rússia, Obninsk, 249036

O. Zhunina

Institute of Molecular Diagnostics

Email: nikita_yabbarov@yahoo.com
Rússia, Moscow, 117149

I. Kondrasheva

Institute of Molecular Diagnostics

Email: nikita_yabbarov@yahoo.com
Rússia, Moscow, 117149

I. Zamulaeva

Tsyb Medical Radiological Research Centre, Branch of the National Medical Research Radiological Center

Email: nikita_yabbarov@yahoo.com
Rússia, Obninsk, 249036

E. Severin

Institute of Molecular Diagnostics

Email: nikita_yabbarov@yahoo.com
Rússia, Moscow, 117149

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