Synthesis of Unsymmetrical Polymethine Cyanine Fluorescent Dyes for Nucleic Acid Analysis by Real-Time PCR

A. N. Natyrov; N. A. Vlasova; I. V. Matvienko; E. M. Volkov; V. M. Bayramov; V. E. Kurochkin; J. I. Alexeev

doi:10.1134/S1068162018040131

Synthesis of Unsymmetrical Polymethine Cyanine Fluorescent Dyes for Nucleic Acid Analysis by Real-Time PCR

Autores: Natyrov A.¹^,2, Vlasova N.², Matvienko I.¹^,2, Volkov E.³, Bayramov V.¹, Kurochkin V.⁴, Alexeev J.¹^,2
Afiliações:
1. All-Russia Research Institute of Agricultural Biotechnology
2. Syntol LLC
3. Faculty of Chemistry
4. Institute for Analytical Instrumentation
Edição: Volume 44, Nº 5 (2018)
Páginas: 562-571
Seção: Article
URL: https://journals.rcsi.science/1068-1620/article/view/229031
DOI: https://doi.org/10.1134/S1068162018040131
ID: 229031

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Resumo

The synthesis of unsymmetrical polymethine cyanine derivatives of f luorescent dyes is described, and their potential as a component of hybridization probes for the real-time PCR technique is first demonstrated.