Experimental Cerebral Ischemia Affects the Expression of Circular RNA Genes of Metabotropic Glutamate Receptors mGluR3 and mGluR5 in Rat Brain
- Autores: Filippenkov I.1, Stavchansky V.1, Denisova A.2, Ivanova K.3, Limborska S.1,2, Dergunova L.1,2
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Afiliações:
- Institute of Molecular Genetics
- Pirogov Russian National Research Medical University
- Faculty of Fundamental Medicine
- Edição: Volume 44, Nº 3 (2018)
- Páginas: 302-309
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228927
- DOI: https://doi.org/10.1134/S1068162018030044
- ID: 228927
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Resumo
Circular RNAs (circRNAs) are a new RNA type that show predominantly brain-specific expression pattern in mammals. Their individual representatives, acting as competitive endogenous RNAs, can bind microRNAs and contribute to the protection of functional transcripts. In the model of transient cerebral ischemia in rats, we studied the expression of mGluR3 and mGluR5 glutamate metabotropic receptor genes (Grm3 and Grm5). These genes are important participants in the metabolic pathways associated with neurosignaling. In the present study, we found that the rat Grm3 and Grm5 genes, in addition to mRNA, encode circRNAs, which are conserved in humans and rodents. In subcortical brain structures of rats containing a lesion focus, the level of these circRNAs is more stable than that of the corresponding mRNA. Using STarMirDB database analysis, the distribution of the microRNA binding sites along the mRNA molecules of human GRM3 and GRM5 genes which are homologous to the corresponding rat genes was elucidated. It has been revealed that sufficiently large number of binding sites is located within exons, which are also part of conservative circRNAs. The results may indicate the functional role of the circRNAs of the investigated genes as competitive endogenous RNAs in the response of brain cells to ischemia.
Sobre autores
I. Filippenkov
Institute of Molecular Genetics
Autor responsável pela correspondência
Email: Filippenkov@img.ras.ru
Rússia, Moscow, 123182
V. Stavchansky
Institute of Molecular Genetics
Email: Filippenkov@img.ras.ru
Rússia, Moscow, 123182
A. Denisova
Pirogov Russian National Research Medical University
Email: Filippenkov@img.ras.ru
Rússia, Moscow, 117997
K. Ivanova
Faculty of Fundamental Medicine
Email: Filippenkov@img.ras.ru
Rússia, Moscow, 119991
S. Limborska
Institute of Molecular Genetics; Pirogov Russian National Research Medical University
Email: Filippenkov@img.ras.ru
Rússia, Moscow, 123182; Moscow, 117997
L. Dergunova
Institute of Molecular Genetics; Pirogov Russian National Research Medical University
Email: Filippenkov@img.ras.ru
Rússia, Moscow, 123182; Moscow, 117997