Chitosan nanoparticles targeted to the tumor-associated ganglioside GD2

Resumo

Methodological approaches to the creation of nanoparticles based on chitosan derivatives and targeted to the GD2-positive tumor cells were developed. The GD2-specific monoclonal antibodies and their Fab-fragments and scFv-fragments were obtained and studied as vector molecules. Various methods for covalent conjugation of these molecules to the nanoparticles were also studied. It was shown that site-specific conjugation of scFv-fragments of GD2-specific antibodies to the chitosan nanoparticles by using a reagent BMPS is the optimal approach to create targeted chitosan-based nanoparticles directed to tumor-associated ganglioside GD2.