Email this article Chitosan nanoparticles targeted to the tumor-associated ganglioside GD2
- Authors: Zubareva A.A.1,2, Boyko A.A.1, Kholodenko I.V.1,3, Rozov F.N.4, Larina M.V.1, Aliev T.K.5, Doronin I.I.1, Vishnyakova P.A.1, Molotkovskaya I.M.1, Kholodenko R.V.1,6
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Affiliations:
- Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Institute of Bioengineering
- Orekhovich Institute of Biomedical Chemistry
- Belozersky Institute of Physicochemical Biology
- Department of Chemistry
- Real Target LLC
- Issue: Vol 42, No 5 (2016)
- Pages: 532-545
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228136
- DOI: https://doi.org/10.1134/S1068162016050150
- ID: 228136
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Abstract
Methodological approaches to the creation of nanoparticles based on chitosan derivatives and targeted to the GD2-positive tumor cells were developed. The GD2-specific monoclonal antibodies and their Fab-fragments and scFv-fragments were obtained and studied as vector molecules. Various methods for covalent conjugation of these molecules to the nanoparticles were also studied. It was shown that site-specific conjugation of scFv-fragments of GD2-specific antibodies to the chitosan nanoparticles by using a reagent BMPS is the optimal approach to create targeted chitosan-based nanoparticles directed to tumor-associated ganglioside GD2.
About the authors
A. A. Zubareva
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Institute of Bioengineering
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997; pr. Leninskii 33, bld. 2, Moscow, 119071
A. A. Boyko
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997
I. V. Kholodenko
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Orekhovich Institute of Biomedical Chemistry
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997; ul. Pogodinskaya 10, str. 8, Moscow, 119121
F. N. Rozov
Belozersky Institute of Physicochemical Biology
Email: khol@mail.ru
Russian Federation, Moscow
M. V. Larina
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997
T. K. Aliev
Department of Chemistry
Email: khol@mail.ru
Russian Federation, Moscow
I. I. Doronin
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997
P. A. Vishnyakova
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997
I. M. Molotkovskaya
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997
R. V. Kholodenko
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Real Target LLC
Author for correspondence.
Email: khol@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, B-437, Moscow, 117997; ul. Miklukho-Maklaya 16/10, Moscow, 117997
