Formation of an Efficient Energy Transfer Complex between Quantum Dots and Exiguobacterium sibiricum Retinal Protein via the Histidine-Cysteine Anchor
- Авторлар: Lukashev E.1, Petrovskaya L.2, Tretyak M.2, Kryukova E.2, Sizova S.2, Oleinikov V.2
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Мекемелер:
- Department of Biology
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Шығарылым: Том 44, № 6 (2018)
- Беттер: 687-694
- Бөлім: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229053
- DOI: https://doi.org/10.1134/S1068162018050114
- ID: 229053
Дәйексөз келтіру
Аннотация
Resonance energy transfer in self-assembled hybrid structures formed by water-soluble semiconductor CdSe/ZnS quantum dots (QD) with a cysteine shell and the Exiguobacterium sibiricum retinal protein (ESR) in three modifications, with and without a 6-membered C-terminal histidine tag, and with both the histidine tag and C-terminal cysteine residue. Steady-state and time-resolved fluorescence spectroscopy was used to demonstrate that nonradiative energy transfer from QD onto the ESR protein depends on the strength of intermolecular interactions in the hybrid complex. A shift from electrostatic interactions in the QD-ESR donor-acceptor pair to coordinate bond with an additional disulfide bond resulted in an increase of the energy transfer efficiency from 40 to over 90%. The proposed method to produce noncovalent conjugates from QDs and light-sensitive proteins can be a promising one for biomedical applications, as well as for the development of new solar energy accumulation systems.
Негізгі сөздер
Авторлар туралы
E. Lukashev
Department of Biology
Email: lpetr65@yahoo.com
Ресей, Moscow, 119992
L. Petrovskaya
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Хат алмасуға жауапты Автор.
Email: lpetr65@yahoo.com
Ресей, Moscow, 117997
M. Tretyak
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: lpetr65@yahoo.com
Ресей, Moscow, 117997
E. Kryukova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: lpetr65@yahoo.com
Ресей, Moscow, 117997
S. Sizova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: lpetr65@yahoo.com
Ресей, Moscow, 117997
V. Oleinikov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: lpetr65@yahoo.com
Ресей, Moscow, 117997